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Principles of mass spectrometry will be provided including description of hardware and software. Basic principles of ion fragmentation will be explained and interpretation of MS spectra generated by electron ionization will be covered in more detail. Current ion sources, analyzators, and detectors will be discussed. Hyphenated techniques, specifically, GC-MS and LC-MS will be covered.
Last update: Pátková Vlasta (05.01.2018)
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R: H.C.Hill: Introduction to Mass Spectrometry, Heyden & Son Ltd, London 1972 A: Fred W. McLafferty, František Tureček: Interpretation of Mass Spectra, University Science Books, Sausalito, California 1993, ISBN 0-935702-25-3, Fourth Edition Last update: Pátková Vlasta (05.01.2018)
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Presentations and excursion within the department. Last update: Pátková Vlasta (05.01.2018)
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1. Principles of mass spectrometry, history of mass spectrometry. 2. Ionization techniques, ion separation and their detection. 3. Sector MS instruments and high resolution. 4. Quadrupole, time-of-flight, and FT MS instruments. 5. Gass chromatography-mass spectrometry (GC-MS), principles. 6. Interpretation of EI spectra of organic molecules, molecular ion, fragments. 7. Fragmentation mechanism, isotopic clusters. 8. EI spectra of selected compounds - hydrocarbons, alcohols, esters, acids, and ethers. 9. EI spectra of selected compounds - compounds containing N, S, B, and metals. 10. Interpretation of real MS spectra, MS libraries, TIC a SIM chromatograms. 11. Liquid chromatography-mass spectrometry, ionization under atmospheric pressure. Last update: Pátková Vlasta (05.01.2018)
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Presentations in electronic format available for students on the intranet. Last update: Pátková Vlasta (05.01.2018)
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Students will know: Basic principles of mass spectrometry including knowledge of hardware and software, knowledge of hyphenated techniques namely GC-MS, LC-MS, basic knowledge of MS spectra interpretation (generated by electron ionization). Last update: Pátková Vlasta (05.01.2018)
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Basic knowledge of analytical chemistry (ACH1 and ACH2) is necessary. Last update: Pátková Vlasta (05.01.2018)
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Basic knowledge of analytical chemistry (ACH1 and ACH2) is necessary. Last update: Pátková Vlasta (05.01.2018)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Účast na přednáškách | 1 | 28 | ||
Příprava na zkoušku a její absolvování | 1 | 28 | ||
2 / 2 | 56 / 56 |