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This course is focused on understanding the principles of modern operating procedures and bioanalytical methods that are used in the analysis of complex biological samples for the determination of various analytes in biological matrix. The basis of this subject is to introduce the student to modern instrumental analytical methods for isolation, identification and determination of biologically important compounds.
Last update: Pátková Vlasta (05.01.2018)
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R: Beds P., Trace Analysis: A structured approach to obtaining reliable results, Editor(s): Prichard E., Points J., Mackay G. M., 1996, ISBN: 978-1-84755-940-1 R: Dean J. R., Methods for environmental trace analysis, John Wiley & Sons, 2003, ISBN: 0-470-84422-1 A: Lobinski R., Marczenko Z., Spectrochemical Trace Analysis for Metals and Metalloids, Elsevier, 1996, ISBN: 978-0-444-82368-7 A: Howard A. G., Statham P. J., Inorganic Trace Analysis: Philosophy and Practice, John Wiley & Sons, 1997, ISBN: 978-0-471-97672-1 Last update: Pátková Vlasta (05.01.2018)
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1. Trace analysis and its significance in modern world. The definition of terms for trace and ultra-trace analyses 2. Difficulties in achieving reliable results at work with small amount of sample; procedures for their solution 3. Determination of sensitivity and detection limits for used methods. Requirements for standards, certified reference materials, chemicals 4. Requirements for process and laboratory equipment in trace analysis. Ways how to reduce contamination of samples 5. Representative sampling and material homogeneity; contamination and loss of analyte during analytical procedure 6. Sample stability and requirements for sample storage 7. Sample preparation in trace and ultra-trace analysis of inorganic and organic analytes. Processes of increasing analyte concentration. Enrichment techniques 8. Derivatization procedures, radioactive and non-radioactive labelling, and selection of suitable technique for determination of micro- and ultramicro amounts of analytes 9. Determination of micro-and ultramicro-amount analytes in biological samples 10. Instrumental methods in trace and ultra-trace analysis 11. Conventional tests and modern methods in trace and ultra-trace analysis 12. Trace analysis of technical samples, waters, airs and gases, foods and agricultural samples. Clinical and forensic analysis 13. Local and surface analyses; trace analysis over large distances 14. Assessment of correctness of the outputs obtained by trace analysis; errors of determination Last update: Pátková Vlasta (05.01.2018)
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Prichard E., MacKay G. M., Points J., Trace Analysis: A Structured Approach to Obtaining Reliable Results (http://books.google.cz/books?isbn=0854044175 ) Last update: Pátková Vlasta (05.01.2018)
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Students will expand and obtain new interdisciplinary knowledge in analytical and bioanalytical chemistry allowing them better orientation in the world of modern chemistry and to find applications in routine bioanalytical laboratories and in various fields of scientific and research activity, too. Last update: Pátková Vlasta (05.01.2018)
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Analytical Chemistry I, II Last update: Pátková Vlasta (05.01.2018)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Konzultace s vyučujícími | 0.5 | 14 | ||
Účast na přednáškách | 1.5 | 42 | ||
Příprava na zkoušku a její absolvování | 2 | 56 | ||
4 / 4 | 112 / 112 |