SubjectsSubjects(version: 923)
Course, academic year 2021/2022
Trace and ultra-trace analysis - M402027
Title: Metody stopové a ultrastopové analýzy
Guaranteed by: Department of Analytical Chemistry (402)
Faculty: Faculty of Chemical Engineering
Actual: from 2019
Semester: summer
Points: summer s.:4
E-Credits: summer s.:4
Examination process: summer s.:
Hours per week, examination: summer s.:2/1, C+Ex [HT]
Capacity: unlimited / unlimited (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type:  
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Sýkora David doc. RNDr. Dr.
Sinica Alla Mgr. Ph.D.
Interchangeability : N402086
Annotation -
Last update: Pátková Vlasta (05.01.2018)
This course is focused on understanding the principles of modern operating procedures and bioanalytical methods that are used in the analysis of complex biological samples for the determination of various analytes in biological matrix. The basis of this subject is to introduce the student to modern instrumental analytical methods for isolation, identification and determination of biologically important compounds.
Aim of the course -
Last update: Pátková Vlasta (05.01.2018)

Students will expand and obtain new interdisciplinary knowledge in analytical and bioanalytical chemistry allowing them better orientation in the world of modern chemistry and to find applications in routine bioanalytical laboratories and in various fields of scientific and research activity, too.

Literature -
Last update: Pátková Vlasta (05.01.2018)

R: Beds P., Trace Analysis: A structured approach to obtaining reliable results, Editor(s): Prichard E., Points J., Mackay G. M., 1996, ISBN: 978-1-84755-940-1

R: Dean J. R., Methods for environmental trace analysis, John Wiley & Sons, 2003, ISBN: 0-470-84422-1

A: Lobinski R., Marczenko Z., Spectrochemical Trace Analysis for Metals and Metalloids, Elsevier, 1996, ISBN: 978-0-444-82368-7

A: Howard A. G., Statham P. J., Inorganic Trace Analysis: Philosophy and Practice, John Wiley & Sons, 1997, ISBN: 978-0-471-97672-1

Learning resources -
Last update: Pátková Vlasta (05.01.2018)

Prichard E., MacKay G. M., Points J., Trace Analysis: A Structured Approach to Obtaining Reliable Results ( )

Syllabus -
Last update: Pátková Vlasta (05.01.2018)

1. Trace analysis and its significance in modern world. The definition of terms for trace and ultra-trace analyses

2. Difficulties in achieving reliable results at work with small amount of sample; procedures for their solution

3. Determination of sensitivity and detection limits for used methods. Requirements for standards, certified reference materials, chemicals

4. Requirements for process and laboratory equipment in trace analysis. Ways how to reduce contamination of samples

5. Representative sampling and material homogeneity; contamination and loss of analyte during analytical procedure

6. Sample stability and requirements for sample storage

7. Sample preparation in trace and ultra-trace analysis of inorganic and organic analytes. Processes of increasing analyte concentration. Enrichment techniques

8. Derivatization procedures, radioactive and non-radioactive labelling, and selection of suitable technique for determination of micro- and ultramicro amounts of analytes

9. Determination of micro-and ultramicro-amount analytes in biological samples

10. Instrumental methods in trace and ultra-trace analysis

11. Conventional tests and modern methods in trace and ultra-trace analysis

12. Trace analysis of technical samples, waters, airs and gases, foods and agricultural samples. Clinical and forensic analysis

13. Local and surface analyses; trace analysis over large distances

14. Assessment of correctness of the outputs obtained by trace analysis; errors of determination

Registration requirements -
Last update: Pátková Vlasta (05.01.2018)

Analytical Chemistry I, II

Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0.5 14
Účast na přednáškách 1.5 42
Příprava na zkoušku a její absolvování 2 56
4 / 4 112 / 112