SubjectsSubjects(version: 855)
Course, academic year 2019/2020
  
Chiroptical methods and analysis of chiral drugs - M402034
Title: Chiroptické metody a analýza chirálních léčiv
Guaranteed by: Department of Analytical Chemistry (402)
Actual: from 2019
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Capacity: unlimited / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type:  
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Záruba Kamil doc. Ing. Ph.D.
Urbanová Marie prof. RNDr. CSc.
Interchangeability : N402102
Annotation -
Last update: Pátková Vlasta (05.01.2018)
The course represents the fundamental concepts of the chiroptical methods and the methods of the enantiomer separation which become important in the pharmacological industry and at the drug characterization because the therapeutic as well as negative drug impacts are connected to the specific enantiomer. The principles and techniques of the different chiral separation methods are explained. The other analytical methods devoted to structural analysis of chiral substances are introduced together with the detection techniques by chiral sensors. The second part of course covers the theoretical basics of chiroptical methods, the concepts of chiroptical instrumental methods and the methodology of chiroptical measurements with special focus to vibrational and electronic circular dichroism. The main application of circular dichroism, their possibilities and limits are described.
Aim of the course -
Last update: Pátková Vlasta (05.01.2018)

Students will be able to:

• Orientate themselves in basic mechanisms of different impact of individual enantiomers on living organism

• Understand the basis of chiral separation and characterize the basic chiral sensors

• Understand principles and techniques of the different methods of chiral separation

• Obtain knowledge of chiroptical methods (theoretical basics, instrumentation techniques, application)

• Detailed knowledge of the spectroscopy of circular dichroism in the both electronic and vibrational variants.

• Orientate themselves in utilization of quantum mechanics method of calculation of chiroptical properties.

Literature -
Last update: Pátková Vlasta (05.01.2018)

A: Analýza chirálních sloučenin (Tesařová E., Ed.), Pražské analytické centrum inovací: Praha, 2007, ISBN: 978-80-86238-23-4.

A: Subramanian G. (Ed.); Chiral Separation Techniques-A Practical Approach; Wiley-VCH, 2001, ISBN: 3-527-29875-4.

A: Francotte E., Lindner W. (Eds); Chirality in Drug Research; Wiley-VCh, 2006, ISBN: 978-3-527-31076-0.

A: Urbanová M., Maloň P. Circular Dichroism Spectroscopy v Analytical Methods in Supramolecular Chemistry (Schalley C. A., Ed.), Wiley VCH: Weinheim, Germany, Second, Completely Revised and Enlarged Edition, 2012, str. 337-369.ISBN: 978-3-527-32982-3.

A: Setnička V., Urbanová M. Spektroskopie cirkulárního dichroismu v Moderní přístupy k farmaceutické analýze (Dohnal J., Jampílek J., Král V., Řezáčová A., Eds.), Farmaceutická fakulta Veterinární a farmaceutické univerzity Brno: Brno, 2010, kapitola 4.6.

Learning resources -
Last update: Pátková Vlasta (05.01.2018)

Analýza chirálních sloučenin (Tesařová E., Ed.), Pražské analytické centrum inovací: Praha, 2007, ISBN: 978-80-86238-23-4.

Syllabus -
Last update: Pátková Vlasta (05.01.2018)

1. Chirality and biological activity

2. Stereoselective intermolecular interaction, chiral selectors, separation by the diastereomer crystallization

3. Liquid chromatography of chiral drugs

4. Other chromatographic methods and electromigration techniques

5. Optimization of chiral separations, preparative separation

6. Structural analysis of chiral drug - NMR, RTG, MS, enantioselective sensors and biosensors

7. Variants of chiroptical methods

8. Interaction of circular polatized light with matter, rotational strength, introduction to theory of circular dichroism

9. Electronic circular dichroism

10. Vibrational circular dichroism, optical rotational dispersion, Raman optical activity

11. Methodology of circular dichroism, instrumentation development

12 Advanced processing of circular dichroism spectra, ab initio calculation of chiroptical properties

13. Application of circular dichroism in conformation analysis of biomolecules, biologically important interactions and supramolecular systems

14. Laboratory of electronic and vibrational circular dichroism

Registration requirements -
Last update: Pátková Vlasta (05.01.2018)

Analytical chemistry I.

Separation methods (or equivalent)

Molecular spectroscopy (or equivalent)

Teaching methods
Activity Credits Hours
Účast na přednáškách 2 56
Příprava na zkoušku a její absolvování 1 28
3 / 3 84 / 84
Coursework assessment
Form Significance
Examination test 100

 
VŠCHT Praha