SubjectsSubjects(version: 953)
Course, academic year 2019/2020
Cultivation Techniques and Modeling of Bioprocesses - N319040
Title: Kultivační techniky a modelování bioprocesů
Guaranteed by: Department of Biotechnology (319)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019 to 2020
Semester: winter
Points: winter s.:7
E-Credits: winter s.:7
Examination process: winter s.:
Hours per week, examination: winter s.:1/7, MC [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Is provided by: M319022
Guarantor: Karabín Marcel Ing. Ph.D.
Is interchangeable with: M319022
Examination dates   Schedule   
Annotation -
The purpose of this course is to acquire complex skills which enable to plan, prepare and carry out experiments and evaluate both on-line and off-line data gained in the process. The data are then used for matematical modelling of these processes.
Last update: TAJ319 (11.12.2013)
Aim of the course -

Students will be able to carry out:

Long term storage of strains, inoculum preparation

Experiments planning, cultivation in shaken flasks and bioreactors, production of primary and/or secondary metabolites

Analytical procedures used in biotechnology

Data evaluation

Modelling of bioprocesses

Last update: TAJ319 (23.08.2013)
Literature -

R: Moo-Young M.: Comprehensive biotechnology, Elsevier B.V., 2011, ISBN: 9780080885049

R: Scragg A.: Environmental Biotechnology, Oxford University Press, 2005, ISBN: 0-19-926867-3

R: Basařová G., Šavel J., Basař P., Lejsek T.: Pivovarství: Teorie a praxe výroby piva, VŠCHT Praha (1. vydání, 2010), ISBN: 978-80-7080-734-7

Last update: TAJ319 (23.08.2013)
Learning resources -

Last update: PAULOVAE (30.07.2013)
Syllabus -

1. Medium and inoculum preparation, long term storage of strains (glycerol stocks, deep freezing, agar plates, freeze drying)

2. Determination of KLa, bioreactor set up, calibration of probes, sterilization

3. Batch and fed-batch cultivation in bioreactor

4. Solid state cultivation

5. Malting process, malt quality evaluation

6. Brewing process, preparation of wort, inoculation, process monitoring and evaluation

7. Continuous cultivation, kinetics of substrate consumption in packed bed bioreactor

8. Photobioreactor - batch and continuous cultivation of microalgae

9. Phase flow characterization

10. Process balancing

11. Programming language and its use in practice

12. Modelling of biotechnological processes

13. Individual project - data evaluation

14. Presentation of induvidual projects

Last update: TAJ319 (23.08.2013)
Registration requirements -

Fundamentals of Bioengineering, Chemical Engineering,

Last update: PAULOVAE (30.07.2013)
Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0.7 20
Účast v laboratořích (na exkurzi nebo praxi) 2 56
Obhajoba individuálního projektu 0.2 6
Účast na přednáškách 0.5 14
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1.3 36
Práce na individuálním projektu 1.3 36
Účast na seminářích 1 28
7 / 7 196 / 196
Coursework assessment
Form Significance
Regular attendance 10
Report from individual projects 30
Defense of an individual project 10
Report from individual projects 30
Continuous assessment of study performance and course -credit tests 20