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Biochemistry: Seminar I is mainly focused on practical knowledge necessary for successful mastery of basic laboratory techniques. It familiarizes students with various separation techniques and immunochemical methods and allows the understanding of their real use in practice using specific examples. In the second half the seminar is focused on natural biocatalysts - enzymes, on their classification, kinetics and inhibition, again using examples from laboratory work.
Last update: Lipov Jan (08.08.2013)
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R: Káš J., Kodíček M., Valentová O.: Laboratorní techniky biochemie. 1. vyd. Vysoká škola chemicko-technologická v Praze, Praha 2006. ISBN 80-7080-586-2 A: Nelson D. L., Cox M. M., Principles of Biochemistry, 4. vydání, W.H.Freeman&Co., New York, 2005, 978-0716762652 Last update: Lipov Jan (08.08.2013)
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1. Introduction 2. Aminoacids (structure, classification, peptide formation) 3. Aminoacids (charge properties, isoelectric point) 4. Aminoacids (titration curves, aminoacids as buffers) 5. Separation techniques Ia (gel permeation and ion exchange chromatography) 6. Separation techniques Ib (other chromatochraphy techniques) 7. Separation techniques II - electrophoresis 8. Immunochemical methods 9. Determination of protein concentration 10. Determination of protein primary structure 11. Enzymes (basic properties, classification to classes, nomenclature) 12. Enzyme kinetics (examples) 13. Enzyme inhibition (examples) 14. Final summary and practice Last update: TAJ320 (26.09.2013)
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Kodíček Milan: Biochemické pojmy : výkladový slovník (http://vydavatelstvi.vscht.cz/knihy/uid_es-002/) Last update: Lipov Jan (08.08.2013)
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Students will be able to: draw formulas and evaluate charge properties of coded aminoacids draw the titration curve of peptides understand basic chromatography techniques (gel permeation, ion exchange, affinity) understand basics of immunochemical methods divide enzymes to the classes understand basics of enzyme kinetics and inhibition Last update: TAJ320 (26.09.2013)
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none Last update: Benešová Eva (26.08.2013)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Příprava na zkoušku a její absolvování | 0.5 | 14 | ||
Účast na seminářích | 0.5 | 14 | ||
1 / 1 | 28 / 28 |
Coursework assessment | |
Form | Significance |
Continuous assessment of study performance and course -credit tests | 100 |