SubjectsSubjects(version: 955)
Course, academic year 2023/2024
Clinical Biochemistry - N320011A
Title: Klinická biochemie
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2022
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: cancelled
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Fusek Martin prof. Ing. CSc.
Krausová Martina doc. Ing. Ph.D.
Is interchangeable with: M320029
Examination dates   Schedule   
Annotation -
Students will learn about several areas in the course of Clinical Biochemistry. The first section will explain the main methodological approaches used human diagnostics. The second part of the lectures focuses on the target analyzed molecules, which are used in the current medicine according to the function of these molecules - such as enzymes, fats, hormones, etc. For each category the function and the use for diagnostics will be explained. The last part of the study focuses on the diagnostics of liver, kidney, and the diagnosis of the internal environment of the organism, including acid-base balance.
Last update: TAJ320 (11.12.2013)
Aim of the course -

Students will know:

the importance of basic molecules in human diagnostics

laboratory methods and instrumental methods for the analysis of these molecules

function and basic pathological processes of major organs and their reflection in the levels of biomarkers

Last update: Krausová Martina (12.08.2013)
Literature -

R: Novák F., Úvod do klinické biochemie, Karolinum, 2002, 8024603667

A: Zima T., Laboratorní diagnostika, Galén, 2007, 9788024614236

A: Koolman J., Röhm K.-H, Barevný atlas biochemie, Grada Publishing, 2012, 978-80-247-2977-0

A: Karlson P., Gerok W., Gross W, Pathobiochemie, Academia, 1987

Last update: Fialová Jana (13.08.2013)
Learning resources -

Students obtain electronical version of materials by email at the beginning of the semester.

Last update: Krausová Martina (12.08.2013)
Syllabus -

1. Introduction

Clinical biochemistry / definition, history, basic tasks / Overview of lectured themes / Basic materials analysed in a clinical laboratory / Quality biochemical analysis / Safety

2. Methods in Clinical Biochemistry I

Separation Methods / Analytical methods / Molecular genetic analysis / Molecular diagnostics

3. Methods in Clinical Biochemistry II

Immunochemical methods / Summarization of the testing - Basic investigated parameters / Specific tests

4. Metabolism of amino acids

Overview of amino acid / Amino acid metabolism / Metabolic disorders and their detection

5. Proteins and clinical biochemistry

Main types and functions of protein / Protein metabolism - synthesis and degradation / Examination protein / Main clinically significant plasma proteins / Proteins recovered in the urine

6. Enzymes - function and disorders

The role of enzymes in the body / Determination of enzymatic activity / Main enzymes in clinical biochemistry and their determination

7. Carbohydrate metabolism and its disorders

Carbohydrates and their metabolism / Physiological functions of carbohydrates / Diabetes and its examination / Other disorders of carbohydrate metabolism

8. Lipids and their metabolism

Structure and physiological functions / Digestion and transport / Apolipoproteins / Enzymes of lipid transport / Atherosclerosis / Biochemical screening

9. Liver functions and related biochemical screening

Liver - description and metabolism / Liver diseases / Analytical procedures for the diagnosis of liver diseases

10. Renal functions and related biochemical screening

Kidney - description and function / Main biomarkers for the diagnosis of renal functions / Analytical Methods

11. Hormones and their analysis

Classification and functions of basic hormone / Clinical applications

12. Tumor markers

Cancer diseases / Classification of tumor markers / Use of tumor markers

13. Electrolytes

Basic terms / Main monitored ions / Sodium and Potassium

14. Acid-base balance

Processes affecting pH / Role of carbon dioxide / pH control / ABB parameters

Last update: Fialová Jana (13.08.2013)
Registration requirements -

Biochemistry I (N320001) or Biochemistry (D320001) or Biochemistry A/B (N320024/N320066)

Last update: Krausová Martina (12.08.2013)
Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na zkoušku a její absolvování 2 56
3 / 3 84 / 84
Coursework assessment
Form Significance
Regular attendance 30
Examination test 30
Oral examination 40