SubjectsSubjects(version: 954)
Course, academic year 2021/2022
Foodborne Pathogens: Laboratory - N320012
Title: Laboratoř mikrobiologického zkoumání potravin
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2021
Semester: summer
Points: summer s.:2
E-Credits: summer s.:2
Examination process: summer s.:
Hours per week, examination: summer s.:0/3, MC [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: cancelled
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Guarantor: Pazlarová Jarmila doc. RNDr. CSc.
Class: Základní laboratoře
Is interchangeable with: AM320028, M320028
Examination dates   Schedule   
Annotation -
The subject targets to the identification and quantification of chosen food-born pathogens according to valid EU ISO norms. The subjekt is also focused on molecular biology and immunochemical methods of identification and to the detail characterization of studied microorganisms.
Last update: TAJ320 (11.12.2013)
Aim of the course -

Students will be able to identify and characterize important foodborn pathogens according to the respctive ISO standards.

Last update: TAJ320 (11.12.2013)
Literature -

Commission Regulation (EC) No 1441/2007 of 5 December 2007 amending Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs

R: Valid ČSN ISO norms

Last update: UST320 (25.09.2013)
Learning resources -


Last update: TAJ320 (25.09.2013)
Syllabus -

1. Safety rules in microbiological laboratory, preparation of sterile medium and instruments.

2. Preparation of defined, selective and complex media.

3. Sampling, homogenization of food samples, dilutions, inoculation.

4. Processing of food samples (ready to eat food- RTF).

5. Processing of food produce for heat treatment.

6. Processing of beverages samples.

7. Determation of total count of aerobic and facultatively anaerobic mezophilic bacteria, determination of coliform bacteria and yeasts and molds.

8. Performance of standard cultivation determination following ISO norms of: Bacillus cereus, Escherichia coli, Staphylococcus aureus.

9. Performance of standard cultivation determination following ISO norms of:Salmonella sp., Listeria monocytogenes.

10. Quantitative evaluation of all performed analysis and their assesssment.

11. Lateral flow immunochemical test for confirmation of Listeria monocytogenes and Salmonella sp.

12. Membrane filtration of water, cultivation and evaluation of samples.

13. Rapid identification methods for the identification of food-born pathogens.

14. Principles of qualitative and quantitative procedures of polymerase chain reaction - PCR for the detection of food-born pathogens. Disposal od all used cultures and media.

Last update: UST320 (23.08.2013)
Registration requirements -

Exam from Microbiology

Last update: UST320 (19.09.2013)
Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1 28
2 / 2 56 / 56
Coursework assessment
Form Significance
Regular attendance 90
Report from individual projects 10