SubjectsSubjects(version: 941)
Course, academic year 2019/2020
Biology: Laboratory - N320015
Title: Laboratoř biologie
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019 to 2021
Semester: summer
Points: summer s.:1
E-Credits: summer s.:1
Examination process: summer s.:
Hours per week, examination: summer s.:0/2, MC [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
For type:  
Old code: BIOL
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Burketová Lenka doc. Ing. CSc.
Kotrba Pavel prof. Ing. Ph.D.
Class: Základní laboratoře
Is interchangeable with: B320004, AB320004
Examination dates   Schedule   
Annotation -
Last update: Kotrba Pavel prof. Ing. Ph.D. (28.06.2013)
The course provides training in the methods of light and fluorescence microscopy, good laboratory practice and rules governing organization of a biological experiment. The course objectives further include: inspection of structure of plant and animal cells and tissues, microscopy and cytochemistry of cellular processes, and analysis of chromosomal basis of heredity.
Aim of the course -
Last update: Kotrba Pavel prof. Ing. Ph.D. (09.07.2013)

Students will be able to:

Describe principles the light and fluorescence microscopy lie in and will be knowledgeable in principal parameters of a light microscope.

Independently prepare microscope specimens, inspect them through the light and fluorescence microscopy, organize an experiment and properly record and interpret the observations.

Better understand organization of the cell, plant and animal tissues, mitosis and chromosomal basis of heredity, which were all in theory subjects of preceding Biology I course.

Literature - Czech
Last update: Kotrba Pavel prof. Ing. Ph.D. (09.11.2012)

Z:Kotrba P., Babůrek I., Knejzlík Z., Návody ke cvičením z biologie, VŠCHT Praha, 2006, 8070806230

D:Benda V., Babůrek I., Kotrba P., Základy biologie, VŠCHT Praha, 2005, 8070805870

Learning resources -
Last update: Kotrba Pavel prof. Ing. Ph.D. (09.07.2013)

Anonymous, Boundless Biology Open Textbook,, free acess at

Syllabus -
Last update: Kotrba Pavel prof. Ing. Ph.D. (09.11.2012)

1. Introduction to Light Microscopy, Fluorescence Microscopy and Other Microscopy Techniques

2. Specimen Preparation for Light Microscopy

3. Microscopy of the Cell, Morphology and Inclusion Bodies of the Cell

4. Microscopy of Organelles of the Cell with Light Microscope

5. Microscopy of Organelles of the Cell using Fluorescence Microscopy

6. Cytochemical Staining of Macromolecules and Detection of Enzyme Activity

7. Osmosis in Cells

8. Cell Types and Intercellular Space in Plant Tissues

9. Anatomy of Stems, Roots and Leaves and Vascular Tissue in Plants

10. Histology � Epithelial and Connective Tissues, Blood Smear

11. Histology � Muscle and Nervous Tissues

12. Cytogenetics � Animal Chromosomes and Karyotype

13. Mitosis in Plant Meristem and Cells of Animal Tissue Culture

14. Exercises in Plant, Animal and Human Genetics

Registration requirements -
Last update: Kotrba Pavel prof. Ing. Ph.D. (09.07.2013)


Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
1 / 1 28 / 28
Coursework assessment
Form Significance
Report from individual projects 30
Continuous assessment of study performance and course -credit tests 70