SubjectsSubjects(version: 941)
Course, academic year 2023/2024
Isolation and Characterisation of Biomacromolecules - N320018
Title: Izolace a charakterizace biomakromolekul
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2021
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Is provided by: B320010
For type:  
Additional information:
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Lipovová Petra doc. Ing. Ph.D.
Holubová Barbora doc. Ing. Ph.D.
Is interchangeable with: B320010, AB320010
Examination dates   Schedule   
Annotation -
Last update: Lipovová Petra doc. Ing. Ph.D. (01.07.2013)
The course focuses on understanding the principles of purification techniques for the isolation of biomolecules. The aim is to teach students how to design a proper procedure for the purification of biomolecules target and characterize it. This knowledge can be used in many fields.
Aim of the course -
Last update: Lipovová Petra doc. Ing. Ph.D. (01.07.2013)

Students will be able to:

Understand the principles of membrane, chromatographic and electrophoretic techniques.

Propose a procedure to optimize protein purification.

They will know the basic techniques of immobilization of proteins and cells.

Literature -
Last update: Lipovová Petra doc. Ing. Ph.D. (10.07.2013)

R: Janson J.C.: Protein Purification, Wiley, New York, 1998, ISBN 0471186260

R: Mikkelsen S.R.: Bioanalytical Chemistry, Wiley, New York, 2004, ISBN 0471544477

Learning resources -
Last update: Lipovová Petra doc. Ing. Ph.D. (26.08.2013)

Syllabus -
Last update: Lipovová Petra doc. Ing. Ph.D. (01.07.2013)

1. Introduction - source of proteins, dezintegration, membrane process

2. Precipitation, extraction, and centrifugation

3. Materials for chromatographic and eletrophoretic methods

4. The basic division and instrumentation

5. Gel-permeation chromatography

6. Ionex chromatography

7. Hydrophobic chromatography and reversed phase chromatography

8. Affinity chromatography

9. Thin layer chromatography, recovery and field of chromatography and determination of proteins concentration

10. Introduction to the electrophoretic methods

11. Types of elektrophoretic methods

12. Immobilisation I

13. Immobilisation II

14. Presentation of project

Registration requirements -
Last update: Lipovová Petra doc. Ing. Ph.D. (01.07.2013)


Teaching methods
Activity Credits Hours
Obhajoba individuálního projektu 0.5 14
Příprava na zkoušku a její absolvování 1 28
2 / 3 42 / 84
Coursework assessment
Form Significance
Regular attendance 10
Report from individual projects 20
Examination test 30
Oral examination 40