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Last update: Lipovová Petra doc. Ing. Ph.D. (01.07.2013)
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Last update: Lipovová Petra doc. Ing. Ph.D. (01.07.2013)
Students will be able to: Understand the principles of membrane, chromatographic and electrophoretic techniques. Propose a procedure to optimize protein purification. They will know the basic techniques of immobilization of proteins and cells.
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Last update: Lipovová Petra doc. Ing. Ph.D. (10.07.2013)
R: Janson J.C.: Protein Purification, Wiley, New York, 1998, ISBN 0471186260 R: Mikkelsen S.R.: Bioanalytical Chemistry, Wiley, New York, 2004, ISBN 0471544477 |
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Last update: Lipovová Petra doc. Ing. Ph.D. (26.08.2013)
http://biomikro.vscht.cz/vyuka/?Predmet=ib |
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Last update: Lipovová Petra doc. Ing. Ph.D. (01.07.2013)
1. Introduction - source of proteins, dezintegration, membrane process 2. Precipitation, extraction, and centrifugation 3. Materials for chromatographic and eletrophoretic methods 4. The basic division and instrumentation 5. Gel-permeation chromatography 6. Ionex chromatography 7. Hydrophobic chromatography and reversed phase chromatography 8. Affinity chromatography 9. Thin layer chromatography, recovery and field of chromatography and determination of proteins concentration 10. Introduction to the electrophoretic methods 11. Types of elektrophoretic methods 12. Immobilisation I 13. Immobilisation II 14. Presentation of project |
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Last update: Lipovová Petra doc. Ing. Ph.D. (01.07.2013)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Obhajoba individuálního projektu | 0.5 | 14 | ||
Příprava na zkoušku a její absolvování | 1 | 28 | ||
2 / 3 | 42 / 84 |
Coursework assessment | |
Form | Significance |
Regular attendance | 10 |
Report from individual projects | 20 |
Examination test | 30 |
Oral examination | 40 |