SubjectsSubjects(version: 916)
Course, academic year 2019/2020
Biochemical Instrumentation and Robotics - N320022
Title: Biochemická instrumentace a robotika
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2013 to 2020
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:0/2, MC [HT]
Capacity: unknown / 30 (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type:  
Guarantor: Kučková Štěpánka doc. Ing. Mgr. Ph.D.
Is interchangeable with: M320002
Examination dates   Schedule   
Annotation -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (11.07.2013)
The course is focused on solving practical problems in the biochemical laboratory. The course summarizes the findings of analytical chemistry, biochemistry and basic robotic systems.
Aim of the course -
Last update: TAJ320 (11.12.2013)

Students will be able:

To solve practical problems that commonly occurring in a biochemical laboratory.

Literature -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (11.07.2013)

R: Kodíček M., Karpenko V., Biofyzikální chemie, Academia, 2000, 80-200-0791-1

R: Káš J., Kodíček M., Valentová O., Laboratorní techniky biochemie, Vysoká škola chemicko-technologická v Praze, 2006, 80-7080-586-2

A: Churáček J. a kol., Analytická separace látek, SNTL, Praha 1990, 80-03-00569-8

Learning resources -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (27.08.2013)


Syllabus -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (11.07.2013)

1. Basic terms (measurements, quantities, characteristics of analytical methods), preparation of distilled water, optical methods

2. Biosensors, potentiometric sensors, the principles of pH measurements and maintenance of electrodes

3. Optical, thermal and piezoelectric transducers, sensors based on sensitive polymer layer, requirements for ideal biosensor

4. Procedures used for sample preparation for analysis, filtration, ultrafiltration, dialysis, sonication, microwave devices

5. Robotics, automation, system miniaturization

6. Separation methods, flow cytometry, centrifuges and centrifugal methods, rotors, field flow fractionation

7. Electrophoresis I: basic terms, separation modes

8. Electrophoresis II: detection, blotting, processing and storage of electropherograms

9. Chromatography I: basic terms, methods, TLC, different types of chromatographs

10. Chromatography II: liquid chromatography, Van Deemter equation, diagnostic of problems with chromatographic separation, gel chromatography

11. Chromatography III: Ion exchange chromatography, sorbents for hydrophobic chromatography, chromatofocusing, chelation ion chromatography

12. Detectors used in chromatography, derivatization of analytes, maintenance rules, selection of appropriate equipment

13. Multidimensional separation techniques in proteomics, combining of different separation techniques and detectors

14. Preparative methods, methods of instruments adjusting, troubleshooting

Registration requirements -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (11.07.2013)

Analytical Chemistry, Biochemistry, Biophysical Chemistry

Course completion requirements -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (11.07.2013)

Successful completion of the written examination.

Teaching methods
Activity Credits Hours
Účast na přednáškách 0,5 14
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0,5 14
Příprava na zkoušku a její absolvování 1 28
2 / 3 56 / 84
Coursework assessment
Form Significance
Examination test 75
Continuous assessment of study performance and course -credit tests 25