SubjectsSubjects(version: 955)
Course, academic year 2019/2020
Labelled Compounds - N342012
Title: Izotopově značené sloučeniny
Guaranteed by: Department of Chemistry of Natural Compounds (342)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2013 to 2020
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: 24 / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Guarantor: Havlíček Libor doc. Ing. CSc.
Is interchangeable with: M342007
Examination dates   Schedule   
Annotation -
The course covers the potential of application of isotopically (using stable as well as radioactive isotope) labeled compounds in chemistry, biology and medicine with special aspects of this discipline (synthesis in ng to mg scale, nomenclature, radiolysis, isotopic effect, hygiene of ionizing radiation)
Last update: TAJ342 (30.09.2013)
Aim of the course -

Students will be able to:

Give the most important applications of labeled compounds in natural sciences research, in drug development, and in practical medicine

Name syntheses of these compounds (with emphasis on both relations and differences from usual synthetic chemistry of organic compounds)

Formulate measures due to radiation hygiene

Last update: TAJ342 (30.09.2013)
Literature -

R: Navrátil O., Hála J., Kopunec R., Macášek F., Mikulaj V., Lešetický L. Nuclear chemistry, Academia, Praha 1992.

A: Evans E. A., Oldham K. G.: Radiochemicals in Biomedicinal Research , John Wiley and Sons

A: Evans E. A., Tritium and its compounds, Butterworths, 2. ed.

A: Murray A., Williams D.L.: Organ. Syntheses with Isotopes

A: Donald G.: Syntheses with Stable Isotopes

A: Thomas A. F.: Deuterium Labeling in Organic Chemistry

Last update: TAJ342 (30.09.2013)
Learning resources -

Lecture notes with formulae and reaction schemes

Last update: TAJ342 (30.09.2013)
Syllabus -

1. Definitions of concepts and quantities in the field of labeled compounds (LC), historical overview, importance of LC, intersections with other disciplines, literature

2. Types of radioactive transformations, kinetics of radioactive transformations, interaction of ionizing radiation (IoR) with matter, other physical principles in LC

3. Dosimetry of IoR (detectors: ionizing, scintillating, integral dosimetry methods)

4. Principles of IoR hygiene. Definition of quantities, basic knowledge of IoR effects on molecule, cell, and organism level, hygiene findings, IoR effects

5. Protection against IoR, LNT basis, ALARA access, lesson from serious nuclear disasters

6. Radio iodine, designation for immune chemical determinations (RIA, IRMA, REA, RRA, FPIA, …)

7. Specialties of LC chemistry (isotopic effect, nomenclature of LC, LC stability, specialties resulting from micro scale synthesis in mg to ng)

8. Overview of application of LC, equivalency of chemical bonds, tautomery, reaction mechanisms, biochemical pathways, internal enzyme transfer, metabolic turns, receptor study, drug development)

9. Hydrogen isotopes labeled compounds. Properties of [3H]-compounds, their synthesis

10. 14C nuclide labeled compounds, 32P or 33P nuclide labeled compounds

11. Labeling with stable nuclides (2H, 13C). Application areas, requirements, synthesis

12. Nuclear medicine - diagnostics (functional diagnostics, "ideal emitter", pure gamma, positron tomography, PET, "shorts", therapy

13. Labeled compounds analysis

14. Chemistry of LC of selected structures (peptides, …)

Last update: TAJ342 (30.09.2013)
Registration requirements -

Organic chemistry I

Last update: TAJ342 (30.09.2013)
Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1 28
Příprava na zkoušku a její absolvování 1 28
3 / 3 84 / 84
Coursework assessment
Form Significance
Regular attendance 20
Continuous assessment of study performance and course -credit tests 30
Oral examination 50