SubjectsSubjects(version: 954)
Course, academic year 2021/2022
Isolation and Separation Methods: Laboratory - N342018
Title: Laboratoř isolačních a separačních metod
Guaranteed by: Department of Chemistry of Natural Compounds (342)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:0/6, MC [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: cancelled
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Guarantor: Raich Ivan doc. Dr. Ing.
Is interchangeable with: M342021
Examination dates   Schedule   
Annotation -
Lab training is designed for works whose result would be few tens of milligrams (ca 50 mg) of pure compound which is a sufficient amount for chemical characterization. The intention is to learn to work with small amounts and with special equipment and labware as is usual for research work isolating compounds of natural origin.
Last update: TAJ342 (30.09.2013)
Aim of the course -

Students will be able to:

Isolate and purify small amounts of compounds from natural materials

Work with labware designed for semi-micro preparative scale

Design procedures and equipment suitable for work with small amounts of compounds

Perform spectral and other characterization from small amounts of isolated compounds

Last update: TAJ342 (30.09.2013)
Literature -

R: Mayo D. W., Pike R. M., Butcher S. S.: Microscale Oganic Laboratory , John Wiley and Sons, Inc., 2nd ed., New York, 1989

Last update: TAJ342 (30.09.2013)
Learning resources -

Raich I., Medonos M.: Instructions for Isolation and separation methods: Laboratory, ICT Prague, 2012

Last update: TAJ342 (30.09.2013)
Syllabus -

1. Labor safety in laboratory, construction of apparatuses for basic operations

2. Isolation of caffeine from tea, extraction, sublimation, melting point

3. Isolation of trehalose from yeast, deproteinization, HPLC verification of product purity

4. Isolation of piperine from black pepper, extraction, decantation, TLC, crystallization

5. Isolation of botulin from birch bark, extraction, crystallization, derivatization

6. Isolation of cinnamaldehyde from cinnamon using steam distillation

7. Isolation of D-glucosamine from crab shells, decalcification, hydrolysis, crystallization

8. GLC analysis of cinnamaldehyde

9. HPLC analysis of trehalose on ion-exchange resin

10. NMR spectra measurements of prepared samples

11. IR spectra measurements of prepared samples

Last update: TAJ342 (30.09.2013)
Registration requirements -

Organic chemistry I

Organic chemistry: Laboratory I

Last update: TAJ342 (30.09.2013)
Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 3 84
Obhajoba individuálního projektu 0.5 14
Práce na individuálním projektu 0.5 14
4 / 4 112 / 112
Coursework assessment
Form Significance
Regular attendance 30
Report from individual projects 60
Defense of an individual project 10