SubjectsSubjects(version: 888)
Course, academic year 2020/2021
Bioanalytical Methods - N402016
Title: Bioanalytické metody
Guaranteed by: Department of Analytical Chemistry (402)
Actual: from 2020
Semester: summer
Points: summer s.:3
E-Credits: summer s.:3
Examination process: summer s.:
Hours per week, examination: summer s.:2/0 Ex [hours/week]
Extent per academic year: 2 [hours]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Is provided by: B320006
For type:  
Guarantor: Setnička Vladimír prof. Ing. Ph.D.
Zídková Jarmila RNDr. CSc.
Last update: Zídková Jarmila RNDr. CSc. (20.08.2013)
Bioanalytical methods are applied to determination of analytes using specific interactions between molecules. Modern bioanalytical techniques provide high specificity of analysis (one of reagents is biopolymer and that is why we talk about biospecificity), but also high sensitivity and low detection limit. Part of lectures will cover enzymatic methods, proteomics, electromigration methods, genetic methods (particularly PCR), immunochemical and other biochemical and microbiological methods.
Aim of the course -
Last update: VED402 (16.12.2013)

Students will be able to:

Choose suitable analytical method with appropriate detection limit.

Apply chosen method for samples of different origin.

Orientate themselves in specialized literature concerning biomolecules issue.

Obtain wide knowledge of bioanalytical methods spectra including the possibility and limits of their application.

Literature -
Last update: VED402 (01.10.2013)

R: Králová B., Rauch P., Fukal L., Bioanalytická chemie, Vydavatelství VŠCHT Praha, 2007, ISBN 80-7080-449-1

R: Káš J., Kodíček M., Valentová O., Laboratorní techniky biochemie, Praha 2005, ISBN 80-7080-586-2

R: Ruml T., Rumlová M., Pačes V., Genové inženýrství, Praha 2002, ISBN 80-7080-499-8

Learning resources -
Last update: Zídková Jarmila RNDr. CSc. (20.08.2013)

Additional list of suggested bibliography and electronic references will be provided within individual lectures.

Last update: TAJ402 (15.04.2010)

1. Bioanalytical methods, practical applications, clinical analysis, medicinal chemistry

2. Basics of combinatorial chemistry, chemical libraries analysis

3. Principles of biopolymer analysis (DNA, proteins, polysacharides)

4. Biosensors, construction, applications

5. Immuno assay methods (ELISA, RIA)

6. Enzymology. Systematic classification of enzymes, enzyme kinetics. Immobilized enzymes.

7. Determination of substrates and metabolites of enzyme reactions

8. Experimental techniques of enzyme methods. Application in clinic analyses and food industry

9. Analysis of important categories of biologically important compounds

10. Chromatography of biomolecules. HPLC, FPLC.

11. Afinity chromatography (incl. HAPLC), methods based on molecular recognition.

12. Electroforetic methods and their importance for analytics of biologically important compounds.

13. Usage of modern analytical methods for biopolymers (NMR, X-ray, MS).

14. Analytical methods in medicinal chemistry for new drug development.

Registration requirements -
Last update: Zídková Jarmila RNDr. CSc. (20.08.2013)

Analytical chemistry I

Biochemistry I

Course completion requirements -
Last update: Zídková Jarmila RNDr. CSc. (20.08.2013)

Oral examination

Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1 28
Příprava na zkoušku a její absolvování 1 28
3 / 3 84 / 84
Coursework assessment
Form Significance
Regular attendance 10
Examination test 90