SubjectsSubjects(version: 882)
Course, academic year 2020/2021
Hyphenated methods for drug analysis - N402062
Title: Spřažené metody pro analýzu léčiv
Guaranteed by: Department of Analytical Chemistry (402)
Actual: from 2013
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Capacity: unlimited / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type:  
Guarantor: Dolenský Bohumil doc. Ing. Ph.D.
Sýkora David doc. Dr. RNDr.
This subject contains the following additional online materials
Annotation -
Last update: Sýkora David doc. Dr. RNDr. (26.09.2016)
Hyphenated methods applied for the analysis of drugs are discussed. Principles, advantages vs disadvantages, and typical applications are provided.
Aim of the course -
Last update: Sýkora David doc. Dr. RNDr. (26.09.2016)

Students will be able:

to propose suitable technique to solve a specific analytical problem.

to consider advantages and disadvantages of the individual proposed solutions.

to interpret EI MS spectra on a basic level.

Literature -
Last update: Sýkora David doc. Dr. RNDr. (26.09.2016)

A: Holčapek M. - (Editor) - Spojení vysokoúčinné kapalinové chromatografie a hmotnostní spektrometrie (HPLC/MS), Univerzita Pardubice, 2001, ISBN: 80-7194-390-8.

A: Štulík K. - (Editor), Analytické separační metody, Univerzita Karlova v Praze - Nakladatelství Karolinum, 2005, ISBN: 80-246-0852-9.

A: Gross J.H. - (Editor), Mass Spectrometry - A textbook, Springer, 2004, ISBN:3-540-40739-1.

A: Albert K. - (Editor), On-Line LC-NMR and Related Techniques, Wiley, 2002, ISBN: 978-0-471-49649-6.

A: Watson J.K.; Sparkman O.D. - (Editors), Introduction to Mass Spectrometry, Wiley, 2007, ISBN:978-0-470-51634-8.

A: Niessen, W.M.A.; Liquid Chromatography - Mass Spectrometry; Marcel Dekker, 1999, ISBN: 0-8247-1936-0.

Learning resources -
Last update: VED402 (16.12.2013)

Electronic version of the presentations available to students.

Teaching methods -
Last update: Sýkora David doc. Dr. RNDr. (26.09.2016)


Syllabus -
Last update: Sýkora David doc. Dr. RNDr. (26.09.2016)

1. General principles of hyphenated methods, basic terms of mass spectrometry (MS).

2. Ion sources for MS.

3. MS Analysers - 1.

4. MS Analysers - 2.

5. Ion detectros.

6. Vacuum systems.

7. HPLC-MS; principles and applications.

8. HPLC-MS; basic principles of mass spectra interpretation.

9. GC-MS; principles and applications.

10. Interpretation of EI spectra - 1.

11. Interpretation of EI spectra - 2.

12. Interpretation of EI spectra - 3.

13. Technique GC x GC.

14. Technique LC x LC.

Registration requirements -
Last update: Sýkora David doc. Dr. RNDr. (18.09.2013)


Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na zkoušku a její absolvování 2 56
3 / 3 84 / 84
Coursework assessment
Form Significance
Examination test 50
Oral examination 50