SubjectsSubjects(version: 941)
Course, academic year 2023/2024
Advanced separation methods for drug analysis - N402064
Title: Pokročilé separační metody analýzy léčiv
Guaranteed by: Department of Analytical Chemistry (402)
Faculty: Faculty of Chemical Engineering
Actual: from 2019
Semester: winter
Points: winter s.:5
E-Credits: winter s.:5
Examination process: winter s.:
Hours per week, examination: winter s.:3/0, Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
For type:  
Guarantor: Sýkora David prof. Dr. RNDr.
Is interchangeable with: M402002
Examination dates   Schedule   
Annotation -
Last update: Sýkora David prof. Dr. RNDr. (18.09.2013)
The goal of the presentations is to broaden and extend knowledge of the students in the area of separation techniques.
Aim of the course -
Last update: VED402 (16.12.2013)

Students will have extensive knowledge of the theory and practice of modern separation techniques.

Literature -
Last update: VED402 (01.10.2013)

A: Introduction to Mass Spectrometry - kniha, J.T. Watson and O.D. Sparkman, Wiley, 2007, ISBN: 978-0-470-51634-8.

A: Mass Spectrometry-A Textbook - kniha, J.H. Gross, 2004, Springer, ISBN: 3-540-40739-1.

A: Introduction to Modern Liquid Chromatography - kniha, L.R. Snyder, J.J. Kirkland and J.W. Dolan, Wiley, 2010, ISBN:978-0-470-16754-0.

A: Gas Chromatography and Mass Spectrometry-A Practical Guide - kniha, O.D. Sparkman, Z.E. Penton, and F.G. Kitson, 2011, Elsevier, ISBN:978-0-12-373628-4.

A: Practical HPLC Method Development - kniha, L.R. Snyder, J.J. Kirkland, J.L. Glajch; Wiley, 1997, ISBN:0-471-00703-X.

A: Chromatographic methods - kniha, A. Braithwaite, F.J. Smith; Blackie Academic & Proffessional, 1996, ISBN:0-7514-0158-7.

A: Gas Chromatography - kniha, I.A. Fowlis; ACOL, Wiley, 1995, ISBN:0-471-95467-5.

A: High Performance Liquid Chromatography - kniha, S. Lindsay; ACOL, Wiley, 1992, ISBN:0-471-93180-2.

A: Chromatography Today - kniha, C.F. Poole, S.K. Poole; Elsevier, 1991, ISBN:0-444-88492-0.

A: Chromatography (6th edition), Fundamentals and Applications of Chromatography and Related Differential Migration Methods, kniha, E. Heftmann, Part A, Elsevier, 2004, ISBN: 0-444-51107-5.

A: Modern Practice of Gas Chromatography - R.L. Grob, E.F. Barry, Wiley, 2004, ISBN: 978-0-471-22983-4.

A: HPLC for Pharmaceutical Scientists, Y.V. Kazakevich, R. LoBrutto, Wiley, 2007, ISBN: 978-0-471-68162-5.

A: Analytická separace látek - kniha, J. Churáček; SNTL, 1990.

A: High Performance Capillary Electrophoresis - firemní příručka, D.N. Heiger, Hewlett-Packard, 1992.

A: Basic Gas Chromatography - kniha, H.M. McNair and J.M. Miller, Wiley, 2009, ISBN: 978-0-470-43954-8.

A: Practical High-Performance Liquid Chromatography - kniha, V.R. Meyer, Wiley, 2010, ISBN: 978-0-470-68218-0.

A: The Troubleshooting and Maintenance Guide for Gas Chromatography - D. Rood, Wiley, 2007, ISBN: 978-0-527-31373-0.

Learning resources -
Last update: Sýkora David prof. Dr. RNDr. (18.09.2013)

Syllabus -
Last update: Sýkora David prof. Dr. RNDr. (26.09.2016)

1. Gas chromatograophy - detailed theory, van Deemter plots.

2. Gas chromatograophy - current trends.

3. High-performance chromatography - detailed theory, kinetic plots.

4. High-performance chromatography - current trends

5. Ultrahigh-performance chromatography.

6. Electrophoresis.

7. Capillary electrophoresis.

8. Separation on chips and high-throughput techniques.

9. Solid phase extraction - current status.

10. Simulated moving bead technology.

11. Supercritical fluid chromatography.

12. Mass spectrometry - 1.

13. Mass spectrometry - 2.

14. Webinars - useful source of information.

Entry requirements -
Last update: Sýkora David prof. Dr. RNDr. (18.09.2013)

Students need to have basic knowledge of the separation methods.

Registration requirements -
Last update: Sýkora David prof. Dr. RNDr. (18.09.2013)

Students need to have basic knowledge of the separation methods.

Teaching methods
Activity Credits Hours
Obhajoba individuálního projektu 0.5 14
Účast na přednáškách 1.5 42
Práce na individuálním projektu 1 28
Příprava na zkoušku a její absolvování 2 56
5 / 5 140 / 140
Coursework assessment
Form Significance
Regular attendance 10
Defense of an individual project 20
Oral examination 70