SubjectsSubjects(version: 950)
Course, academic year 2021/2022
Advanced bioanalytical methods - N402068
Title: Pokročilé bioanalytické metody
Guaranteed by: Department of Analytical Chemistry (402)
Faculty: Faculty of Chemical Engineering
Actual: from 2019 to 2021
Semester: summer
Points: summer s.:3
E-Credits: summer s.:3
Examination process: summer s.:
Hours per week, examination: summer s.:2/0, Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
For type:  
Guarantor: Setnička Vladimír prof. Ing. Ph.D.
Zídková Jarmila RNDr. CSc.
Is interchangeable with: M402023
Examination dates   Schedule   
Annotation -
Last update: ZIDKOVAJ (04.10.2013)
Bioanalytical methods are applied to determination of analytes using specific interactions between molecules. Modern bioanalytical techniques provide high specificity of analysis (one of reagents is biopolymer and that is why we talk about biospecificity), but also high sensitivity and low detection limit. Part of lectures will cover enzymatic methods, proteomics, metabolomics, electromigration methods, advanced genetic methods, immunochemical and other biochemical and microbiological methods including modern trends as clinical biodiagnostics and modern individualized therapy approach.
Aim of the course -
Last update: VED402 (16.12.2013)

Students will be able to:

Choose suitable special analytical method with appropriate detection limit.

Apply the chosen method to samples of different origin and their characterization.

Orientate themselves in specialized literature concerning biomolecules issue and special approach to their study.

Obtain wide knowledge of most modern bioanalytical methods spectra including possibility, suitability and limits of their application.

Literature -
Last update: VED402 (04.10.2013)

R: Králová B., Rauch P., Fukal L., Bioanalytická chemie, Vydavatelství VŠCHT Praha, 2007, ISBN 80-7080-449-1

R: Káš J., Kodíček M., Valentová O., Laboratorní techniky biochemie, Praha 2005, ISBN 80-7080-586-2

R: Ruml T., Rumlová M., Pačes V., Genové inženýrství, Praha 2002, ISBN 80-7080-499-8

R: Daussant J., Desvaux F.-X., Introduction to immunochemical techniques for medical diagnosis, food quality control and environmental testing, 2007, ICT Press, 9788070806418

A: Fukal L. - (Editor), Bioafinitní metody, Vysoká škola chemicko-technologická v Praze, Vydavatelství VŠCHT, 2005, ISBN: 80-7080-584-6.

A: Gault v., McClenaghan N., Understanding Bioanalytical Chemistry: Principles and Applications, Wiley, 2009, ISBN: 978-0-470-02906-0.

A: Racek J., Klinická biochemie, Galén 2006, ISBN 80-7262-324-9

Learning resources -
Last update: ZIDKOVAJ (04.10.2013)

Additional list of suggested bibliography and electronic references will be provided within individual lectures.

Syllabus -
Last update: ZIDKOVAJ (04.10.2013)

1. Introduction to bioanalytical methods.

2. Analysis and quantification of biomoleculs.

3. Heavy metals and their influence on health.

4. Proteomics.

5. Metabolomics.

6. Mass spectrometry in bioanalysis.

7. Chromatography of biomolecules II.

8. Principles and application of electrophoresis.

9. Genetic methods II.

10. Recombinant peptides and proteins.

11. Immunochemical techniques II.

12. Tissue culture and flow cytometry.

13. Clinical biodiagnostics, modern individualized therapy.

14. Future direction of bioanalytical methods.

Registration requirements -
Last update: ZIDKOVAJ (04.10.2013)

Analytical chemistry I

Biochemistry I

Bioanalytical methods N402016 (N320006)

Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na zkoušku a její absolvování 2 56
3 / 3 84 / 84
Coursework assessment
Form Significance
Regular attendance 20
Oral examination 80