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Within the course, modern separation techniques such as high-performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC) will be presented and discussed. Their utilization for characterization and purification of biologically active substances will also be presented. Hyphenation of HPLC and SFC with mass spectrometry (MS) for the characterization of organic substances will be emphasized. Theoretical knowledge of spectroscopic methods gained within previous studies will be deepened. The substantial part of the course will be practical demonstration of modern instruments (NMR, HPLC-MS, SFC-MS, preparative HPLC, etc.). Students will fructify the obtained theoretical knowledge and practical skills in solving a special project, which consists of identification of a substance in an unknown sample and the determination of its structure.
Last update: Kohout Michal (23.05.2022)
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Students will be able to: characterize basic properties of an organic substance develop chromatographic methods for characterization and purification of substances using achiral and chiral stationary phases utilize chromatographic methods in hyphenation with mass spectrometry to identify organic substances determine the structure of organic compounds using spectroscopic methods
Last update: Kohout Michal (23.05.2022)
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To finish the subject, it is required to present a method for determination of the unknow sample structure. Last update: Kohout Michal (23.05.2022)
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Williams D.H., Fleming I.: Spectroscopic methods in organic chemistry. 6. edition. ISBN-13: 978-0077118129
Richards S.A., Hollerton J.C.: Essential Practical NMR for Organic Chemistry, 2011 John Wiley & Sons, ISBN: 978-0-470-71092-0 https://onlinelibrary.wiley.com/doi/book/10.1002/9780470976401
Meyer V.R.: Practical High-Performance Liquid Chromatography, 5. edition, Wiley 2010, ISBN-13: 978-0470682173 Last update: Kohout Michal (23.05.2022)
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1.Methods of structural analysis in characterization of biologically active substances 2.Identification of unknown substances: sample preparation and design of experiment 3.Basic properties of substances by means of thin layer chromatography 4.Introduction to high-performance liquid chromatography, screening in normal and reversed phase 5.Preparative chromatography in normal and reversed phase – method development 6.Special chromatographic modes – HILIC, ion-exchange, multimodal stationary phases 7.Supercritical fluid chromatography and its application in pharmaceutical industry 8.Chiral stationary phases, their applications and limitations in analysis and purification of drugs 9.Preparative chiral separation – method development 10.Liquid chromatography hyphenated to mass spectrometry 11.Identification of compound structure by NMR spectroscopy 12.Determination of absolute configuration of enantiomers by means of chiroptic methods 13.Structure determination of an unknown substance 14.Presentation of the determination of the unknown structure Last update: Kohout Michal (23.05.2022)
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