Purification and identification methods in drug design and development - P110026
Title: Čistící a identifikační metody ve výzkumu a vývoji farmak
Guaranteed by: Department of Organic Chemistry (110)
Faculty: Faculty of Chemical Technology
Actual: from 2022
Semester: summer
Points: summer s.:0
E-Credits: summer s.:0
Examination process: summer s.:
Hours per week, examination: summer s.:3/0, other [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Level:  
Note: course is intended for doctoral students only
can be fulfilled in the future
Guarantor: Kohout Michal prof. Ing. Ph.D.
Examination dates   
Annotation -
Within the course, modern separation techniques such as high-performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC) will be presented and discussed. Their utilization for characterization and purification of biologically active substances will also be presented. Hyphenation of HPLC and SFC with mass spectrometry (MS) for the characterization of organic substances will be emphasized. Theoretical knowledge of spectroscopic methods gained within previous studies will be deepened. The substantial part of the course will be practical demonstration of modern instruments (NMR, HPLC-MS, SFC-MS, preparative HPLC, etc.). Students will fructify the obtained theoretical knowledge and practical skills in solving a special project, which consists of identification of a substance in an unknown sample and the determination of its structure.
Last update: Kohout Michal (23.05.2022)
Aim of the course -

Students will be able to:

characterize basic properties of an organic substance

develop chromatographic methods for characterization and purification of substances using achiral and chiral stationary phases

utilize chromatographic methods in hyphenation with mass spectrometry to identify organic substances

determine the structure of organic compounds using spectroscopic methods

Last update: Kohout Michal (23.05.2022)
Course completion requirements -

To finish the subject, it is required to present a method for determination of the unknow sample structure.

Last update: Kohout Michal (23.05.2022)
Literature -

Williams D.H., Fleming I.: Spectroscopic methods in organic chemistry. 6. edition. ISBN-13: 978-0077118129

Richards S.A., Hollerton J.C.: Essential Practical NMR for Organic Chemistry, 2011 John Wiley & Sons, ISBN: 978-0-470-71092-0

https://onlinelibrary.wiley.com/doi/book/10.1002/9780470976401

Meyer V.R.: Practical High-Performance Liquid Chromatography, 5. edition, Wiley 2010, ISBN-13: 978-0470682173

Last update: Kohout Michal (23.05.2022)
Syllabus -

1.Methods of structural analysis in characterization of biologically active substances

2.Identification of unknown substances: sample preparation and design of experiment

3.Basic properties of substances by means of thin layer chromatography

4.Introduction to high-performance liquid chromatography, screening in normal and reversed phase

5.Preparative chromatography in normal and reversed phase – method development

6.Special chromatographic modes – HILIC, ion-exchange, multimodal stationary phases

7.Supercritical fluid chromatography and its application in pharmaceutical industry

8.Chiral stationary phases, their applications and limitations in analysis and purification of drugs

9.Preparative chiral separation – method development

10.Liquid chromatography hyphenated to mass spectrometry

11.Identification of compound structure by NMR spectroscopy

12.Determination of absolute configuration of enantiomers by means of chiroptic methods

13.Structure determination of an unknown substance

14.Presentation of the determination of the unknown structure

Last update: Kohout Michal (23.05.2022)
Learning resources -

https://onlinelibrary.wiley.com/doi/book/10.1002/9780470988749

https://onlinelibrary.wiley.com/doi/book/10.1002/9781118647042

https://onlinelibrary.wiley.com/doi/book/10.1002/9783527611737

https://onlinelibrary.wiley.com/doi/book/10.1002/9780471980582

https://onlinelibrary.wiley.com/doi/book/10.1002/9781118120187

https://onlinelibrary.wiley.com/doi/book/10.1002/0471690082

https://onlinelibrary.wiley.com/doi/book/10.1002/9783527654703

https://onlinelibrary.wiley.com/doi/book/10.1002/0470079096

https://onlinelibrary.wiley.com/doi/book/10.1002/9783527611973

https://onlinelibrary.wiley.com/doi/book/10.1002/9783527809202

https://onlinelibrary.wiley.com/doi/book/10.1002/9780470516898

https://onlinelibrary.wiley.com/doi/book/10.1002/0471736589

https://onlinelibrary.wiley.com/doi/book/10.1002/9780470395813

Last update: Kohout Michal (23.05.2022)