Selected methods of instrumental analysis - P215005
Title: Vybrané metody instrumentální analýzy
Guaranteed by: Department of Sustainable Fuels and Green Chemistry (228)
Faculty: Faculty of Environmental Technology
Actual: from 2023
Semester: both
Points: 0
E-Credits: 0
Examination process:
Hours per week, examination: 3/0, other [HT]
Capacity: winter:unlimited / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Level:  
Note: course is intended for doctoral students only
can be fulfilled in the future
you can enroll for the course in winter and in summer semester
Guarantor: Šimáček Pavel doc. Ing. Ph.D.
Classification: Chemistry > Analytical Chemistry
Is interchangeable with: AP215005
Examination dates   
Annotation -
The subject significantly extends the theoretical and practical knowledge of infrared and Raman spectroscopy, mass spectrometry, liquid and gas chromatography, nuclear magnetic resonance spectroscopy and other method of instrumental analyses. Emphasis is given on applying these methods to samples of crude oil fractions, biofuels, all components of the environment and the correct interpretation of the results. The subject is taught modularly according to the chosen analytical method. Student will choose at least two of the four offered modules.
Last update: Blažek Josef (02.11.2018)
Aim of the course -

Students will gain knowledge about the use of the selected analytical methods for the needs of their own scientific projects.

Last update: Blažek Josef (02.11.2018)
Course completion requirements -

Individual project development

Successful completion of the final written test

Last update: Blažek Josef (02.11.2018)
Literature -

Recommended:

  • Fanali, Salvatore. Liquid chromatography.. Waltham: Elsevier, 2013, https://vufind.techlib.cz/Record/000965836 s. ISBN 978-0-12-415806-1.

  • N. Draffin. An Introduction to Fuel Analysis. United Kingdom: Petrospot Limited, 2009, s. ISBN 978-0-9548097-3-7.

  • Harvey, David. Modern analytical chemistry. Boston: McGraw-Hill, 2000, https://vufind.techlib.cz/Record/000914246 s. ISBN 0-07-237547-7.

  • Gross, Jürgen H., Roepstorff, Peter. Mass spectrometry, a textbook. Berlin: Springer, 2011, https://vufind.techlib.cz/Record/000977132 s. ISBN 978-0-12-415807-8.

  • K. Dettmer-Wilde, W. Engewald . Practical Gas Chromatography: A Comprehensive Reference. Berlin: Springer, 2014, s. ISBN 978-3-642-54639-6.

  • M. Popl, J. Fähnrich. Analytická chemie životního prostředí. Praha: VŠCHT Praha , 1999, s. ISBN 80-7080-336-3.

  • P.R. Griffiths, J.A. de Haseth. Fourier Transform Infrared Spectroscopy. USA: John Wiley a Sons, 2007, s. ISBN 978-0-471-194040.

  • Eckertová, Ludmila. Metody analýzy povrchů, elektronová mikroskopie a difrakce. Praha: Academia, 1996, https://vufind.techlib.cz/Record/000016832 s. ISBN 80-200-0329-0.

Last update: Šimáček Pavel (29.08.2024)
Requirements to the exam -

None

Last update: Blažek Josef (02.11.2018)
Syllabus -

The subject is divided into 4 modules (blocks) according to the dominant method. Part of each module is the elaboration of a project, which will consist of analysis of samples, selected by student or delivered by lecturer, by selected methods and analysis of the results obtained.

Block I - Infrared and Raman spectrometry.

Calculation methods, modelling spectra, visualization of spectra, work with databases, Internet sources of spectral information, mathematical processing of qualitative and qualitative analyses. Techniques for measuring spectra of organic and inorganic samples, different states, differences in the measurement of chemical individuals and mixtures, qualitative analysis, quantitative analysis and preparation of calibration samples. Interpretation of spectra, mutual correlation of spectral information. Experimental solution to the problem, analysis plan, choice of combination of analytical methods for solving the problem, final report and presentation of results.

Block II. Mass spectrometry

Principles, instrumentation, GC-MS and LC-MS connection, direct input to the spectrometer source, sample preparation and own measurements (low, high resolution), qualitative and quantitative analysis, evaluation of results and interpretation of spectra, work with databases, characterization of the structure of the molecule (EI +70 eV, MS-MS), analysis of mixtures of organic substances, development of GC-MS and LC-MS methods, examples of use, experimental solution of the problem, final project and presentation of results.

Block III. Nuclear magnetic resonance

Principle and NMR instrumentation. NMR techniques (1H, 13C, two-dimensional NMR, NMR tomography), sample measurement, noise minimization. Interpretation of spectra, chemical shift, simple spectra, interpretation of 1H spectra of complex hydrocarbon samples, biodiesel spectrum, 13C NMR spectra of complex hydrocarbon samples. Use of NMR data for complex analysis of complex hydrocarbon mixtures (Brown-Ladner, Clutter, Williams). Experimental solution of the problem (structural characterization of products of preparative group analysis of high boiling petroleum fractions), plan of analysis, choice of combination of analytical methods for solution of the problem, final report and presentation of results.

Block IV. Atomic absorption, emission and X-ray spectrometry

Principle and instrumentation of atomic absorption spectrometry. Principle and instrumentation of optical emission spectrometry. Principle and instrumentation of X-ray fluorescence spectroscopy and X-ray diffraction. Use of AAS, ICP OES and XRF for fuel analysis (sulfur, manganese, iron). Use of AAS, ICP OES, XRF and XRD for analysis and characterization of catalysts (usability for elementary phase analysis, matrix influence, interference of determination). Experimental solution of own problem (analysis of selected samples, comparison of results obtained by various methods), analysis plan, choice of combination of analytical methods for solving the given problem, final report and presentation of results.

Last update: Blažek Josef (02.11.2018)
Entry requirements -

Basic knowledge of organic and inorganic chemistry, analytical chemistry, physical chemistry, structural analysis

Last update: Blažek Josef (02.11.2018)
Registration requirements -

None

Last update: Blažek Josef (02.11.2018)