SubjectsSubjects(version: 953)
Course, academic year 2023/2024
Advanced imaging techniques - P320007
Title: Pokročilé zobrazovací techniky
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2022
Semester: summer
Points: summer s.:0
E-Credits: summer s.:0
Examination process: summer s.:
Hours per week, examination: summer s.:3/0, other [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course is intended for doctoral students only
can be fulfilled in the future
Guarantor: Zelenka Jaroslav doc. Ing. Ph.D.
Is interchangeable with: AP320007
This subject contains the following additional online materials
Annotation -
Microscopy techniques provide an efficient and unique approach to the study of fixed tissues and living cells with high specificity and sensitivity. Workshop aims to introduce the students to modern microscopy techniques for biology object imaging. Part of the workshop will be the introduction to automated wide-field, confocal and superresolution fluorescent microscopy. First, a brief introduction to the physical mechanisms underlying these imaging techniques will be given. Then we introduce the theoretical and/or practical basis of particular advanced imaging techniques. The basics of image analysis will be covered as well.
Last update: Lipov Jan (02.05.2018)
Aim of the course -

Student will be introduced to modern facts and methodology in advanced microscopy of biological objects, she/he will obtain an overview of possible techniques, which allows her/him more qualified decisions in scientific research activities.

Last update: Lipov Jan (02.05.2018)
Literature -

Scientific papers and online books from the portfolio of Nature Research, AAAS and others.

Reviews in Fluorescence, Geddes, Chris D., Lakowicz, Joseph R., Springer, ISSN: 1573-8086

Fluorescence Imaging and Biological Quantification, Raquel Seruca, Jasjit S. Suri, J. Miquel Sanches, CRC Press, ISBN: 9781498737043

Fluorescence Microscopy: From Principles to Biological Applications, 2nd Edition,Ulrich Kubitscheck, Wiley-Blackwell, ISBN: 978-3-527-33837-5

Advanced Fluorescence Microscopy Methods and Protocols, Verveer, Peter J., Springer, ISBN: 978-1-4939-2080-8

Super-resolution microscopy collection, Nature

web pages of microscopic companies (Zeiss, Leica, Olympus, Nikon), for example

Last update: Lipov Jan (19.10.2018)
Teaching methods -

Lectures, professional debate, self-study, excursion.

Last update: Lipov Jan (13.09.2018)
Syllabus -

Fluorescence microscopy as a specific part of optical microscopy, quantification of fluorescence, fluorescent tags.

Confocal, multiphoton and correlation microscopy.

The application of fluorescence microscopy - FRAP (Fluorescence Recovery After Photobleaching), FRET (Förster Resonance Energy Transfer)

The application of fluorescence microscopy - TIRF (Total Internal Reflection Fluorescence), SIM (Structured Illumination Microscopy)

The application of fluorescence microscopy - PALM (PhotoActivated Localization Microscopy), STORM (Stochastic Optical Reconstruction Microscopy)

The application of fluorescence microscopy - STED (Stimulated Emission Depletion microscopy), FLIM (Fluorescence-Lifetime Imaging Microscopy).

Image analysis – parameter ajdustment before acquisition, software for image processing and analysis – deconvolution, 3D visualization, colocalization, tracking, correction of chromatic abberations.

Last update: Lipov Jan (02.05.2018)
Entry requirements -

Basic knowledge in the field of optics and microscopic techniques.

Last update: Lipov Jan (02.05.2018)
Course completion requirements -

Active presence of the student on the lectures.

More then 50% from the final test.

Last update: Lipov Jan (02.05.2018)