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Last update: Vlčková Martina Ing. (19.07.2018)
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Last update: Vlčková Martina Ing. (23.07.2018)
Students will understand the principles of modern separation and detection techniques based on high-resolution gas and liquid chromatography with different types of mass spectrometric detectors. They will get the knowledge of mass spectra interpretation, and learn about different softwares used for analytes identification and quantification. They will be able to implement target analyzes and non-target screening, taking into account available analytical instrumentation, and will have the knowledge necessary to properly evaluate and interpret the measured data. |
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Last update: Stránská Milena doc. Ing. Ph.D. (15.11.2022)
Pradip K. Ghosh: Introduction to Protein Mass Spectrometry, 2015, ISBN: 978-0-12-802102-6, Elsevier
Mike S. Lee: Mass Spectrometry Handbook, 2012, ISBN:9780470536735, Wiley
Vitha, MF: Chromatography - Principles and Instrumentation, 2016, ISBN: 9781119270881, Wiley
Z: Kind T., Tsugawa H., Cajka T., Ma Y., Lai Z., Mehta S.S., Wohlgemuth G., Barupal D.K., Showalter M.R., Arita M., Fiehn O.: Identification of small molecules using accurate mass MS/MS search. Mass Spec Rev. 2017;9999:1–20. |
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Last update: Vlčková Martina Ing. (19.07.2018)
https://massbank.eu/MassBank/ https://www.mzcloud.org/ https://chemdata.nist.gov/ www.bio-rad.com/en-cn/product/mass-spectral-databases |
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Last update: Vlčková Martina Ing. (19.07.2018)
Lectures |
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Last update: Vlčková Martina Ing. (18.07.2018)
None. |
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Last update: Vlčková Martina Ing. (18.07.2018)
1. General requirements for the quality of data generated by chromatographic and mass spectrometric techniques; definition of basic concepts. 2. Gas chromatography coupled with mass spectrometry, ionization techniques in GC-MS, conventional detectors. 3. Liquid chromatography coupled with mass spectrometry, ionization techniques, conventional detectors. 4. Mass spectrometry (MS), types of mass spectrometers, acquisition modes. 5. Interpretation of GC-MS mass spectra. 6. Interpretation of LC-MS mass spectra. 7. Mass spectrometry data in food analysis. 8. Library spectra, retention indices. 9. Spectral deconvolution, software for identifying / interpreting analytes. 10. Application of non-target screening in food analysis. 11. Overview and application of different quantification techniques. 12. Criteria for confirmation in mass spectrometry. 13. Significant statistical methods for data processing. 14. Software for automatic data processing. |
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Last update: Vlčková Martina Ing. (18.07.2018)
None. |
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Last update: Vlčková Martina Ing. (18.07.2018)
None |
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Last update: Vlčková Martina Ing. (23.07.2018)
oral exam, passing the test |