SubjectsSubjects(version: 953)
Course, academic year 2019/2020
Recent separation methods with mass-spectrometry detection - P342005
Title: Moderní separační metody s hmotnostní detekcí
Guaranteed by: Department of Chemistry of Natural Compounds (342)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019 to 2019
Semester: both
Points: 0
E-Credits: 0
Examination process:
Hours per week, examination: 3/0, other [HT]
Capacity: winter:unknown / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: can be fulfilled in the future
you can enroll for the course in winter and in summer semester
Guarantor: Lapčík Oldřich prof. Dr. RNDr.
Prokudina Elena Ing. Ph.D.
Cihlářová Petra PharmDr. Ph.D.
Is interchangeable with: AP342005
Examination dates   Schedule   
Annotation -
The course is focussed on a deeper understanding of analytical liquid chromatgraphy and gas chromatography, with the emphasis on combination with mass spectrometer as a detector. Students will be acquainted with the theoretical principles of liquid and gas chromatography, optimization of both methods, practical examples and interpretation of mass spectra.
Last update: Lapčík Oldřich (30.06.2018)
Aim of the course -

Students will understand both the theoretical basis and the practical aspects of liquid and gas chromatography combined with mass spectrometry. They will be able to solve and develop tasks related to the optimization of gas and liquid chromatographic separation and their subsequent detection and interpretation of mass spectra.

Last update: Lapčík Oldřich (30.06.2018)
Literature -

McMaster M.C.: GC/MS – A practical guide, John Wiley, 2007

Sparkman O.D., Penton Z.E., Kitson F.G.: Gas Chromatography and Mass Spectrometry : A Practical Guide, 2nd Edition, Academic Press, 2011

Smith R.M.: Understanding Mass Spectra: A Basic Approach, 2nd Edition, John Wiley & Sons, 2005.

Kromidas S.: The HPLC-MS handbook for practitioners. Weinheim: Wiley-VCH Verlag GmbH & Co., 2017

Last update: Lapčík Oldřich (30.06.2018)
Teaching methods -


study of recommended literature

seminars of course participants


Last update: Lapčík Oldřich (30.06.2018)
Syllabus -

The course deals with the theoretical principles of chromatography, such as thermodynamic and kinetic theory, elution characteristics, integration, mechanisms of separation. These theoretical foundations are followed by instrumentation in gas and liquid chromatography. The attention is paid to the respective types of detectors. The practical part deals with: a) GC - modification of carrier gas, overview of stationary phases, characteristics of capillary columns and principles of their treatment, temperature program, derivatization; b) LC - selection of stationary and mobile phases, optimizing parameters and optimization of the methods, gradient chromatography.

The other part of the course deals with mass detection: theory, instrumentation, types of ion sources and analyzers, ionization methods, combination of LC/MS and GC/MS, interpretation of the mass spectra. Finally, the course deals with the problematics of the experiment run and also practical examples

Last update: Lapčík Oldřich (30.06.2018)
Entry requirements -


Last update: Lapčík Oldřich (30.06.2018)
Registration requirements -


Last update: Lapčík Oldřich (30.06.2018)
Course completion requirements -

The course is finalized with an exam (written and oral).

Last update: Lapčík Oldřich (30.06.2018)
Coursework assessment
Form Significance
Regular attendance 40
Examination test 20
Oral examination 40