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Last update: Lapčík Oldřich prof. Dr. RNDr. (30.06.2018)
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Last update: Lapčík Oldřich prof. Dr. RNDr. (30.06.2018)
Students will understand both the theoretical basis and the practical aspects of liquid and gas chromatography combined with mass spectrometry. They will be able to solve and develop tasks related to the optimization of gas and liquid chromatographic separation and their subsequent detection and interpretation of mass spectra. |
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Last update: Lapčík Oldřich prof. Dr. RNDr. (30.06.2018)
McMaster M.C.: GC/MS – A practical guide, John Wiley, 2007 Sparkman O.D., Penton Z.E., Kitson F.G.: Gas Chromatography and Mass Spectrometry : A Practical Guide, 2nd Edition, Academic Press, 2011 Smith R.M.: Understanding Mass Spectra: A Basic Approach, 2nd Edition, John Wiley & Sons, 2005. Kromidas S.: The HPLC-MS handbook for practitioners. Weinheim: Wiley-VCH Verlag GmbH & Co., 2017 |
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Last update: Lapčík Oldřich prof. Dr. RNDr. (30.06.2018)
lectures study of recommended literature seminars of course participants consultations |
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Last update: Lapčík Oldřich prof. Dr. RNDr. (30.06.2018)
The course deals with the theoretical principles of chromatography, such as thermodynamic and kinetic theory, elution characteristics, integration, mechanisms of separation. These theoretical foundations are followed by instrumentation in gas and liquid chromatography. The attention is paid to the respective types of detectors. The practical part deals with: a) GC - modification of carrier gas, overview of stationary phases, characteristics of capillary columns and principles of their treatment, temperature program, derivatization; b) LC - selection of stationary and mobile phases, optimizing parameters and optimization of the methods, gradient chromatography.
The other part of the course deals with mass detection: theory, instrumentation, types of ion sources and analyzers, ionization methods, combination of LC/MS and GC/MS, interpretation of the mass spectra. Finally, the course deals with the problematics of the experiment run and also practical examples |
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Last update: Lapčík Oldřich prof. Dr. RNDr. (30.06.2018)
None |
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Last update: Lapčík Oldřich prof. Dr. RNDr. (30.06.2018)
None |
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Last update: Lapčík Oldřich prof. Dr. RNDr. (30.06.2018)
The course is finalized with an exam (written and oral). |