SubjectsSubjects(version: 949)
Course, academic year 2021/2022
Bioreactors - P409003
Title: Bioreaktory
Guaranteed by: Department of Chemical Engineering (409)
Faculty: Faculty of Chemical Engineering
Actual: from 2019 to 2022
Semester: both
Points: 0
E-Credits: 0
Examination process:
Hours per week, examination: 3/0, other [HT]
Capacity: winter:unlimited / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
For type: doctoral
Note: course is intended for doctoral students only
can be fulfilled in the future
you can enroll for the course in winter and in summer semester
Guarantor: Přibyl Michal prof. Ing. Ph.D.
Is interchangeable with: AP409003
This subject contains the following additional online materials
Annotation -
Last update: Přibyl Michal prof. Ing. Ph.D. (14.11.2018)
The course serves to gain a broader experience in bioreactor problematics. Students will become familiar with cell structure and functions, basic cellular processes, cellular energetics, types of cellular products, etc. In the next part the kinetics of enzyme reactions - enzyme inhibition, single- and multi-substrate kinetics, kinetics of allosteric enzymes and enzyme cascades are discussed. Various models of microbial growth are introduced as well as different types of microbial interactions. Students will be skilled to create of mathematical models of different types of bioreactors with free enzymes, immobilized enzymes and cells. Particular emphasis is placed on the description of mass and heat transport and reaction processes in multi-phase fermenters. Another part of the course is devoted to the overview of the processes for obtaining of cellular products. Finally, the principles of selected DNA and protein technologies, such as DNA sequencing methods, DNA amplification, preparation of recombinant proteins or mono / polyclonal antibodies or principles of immunoassay are presented. The course is terminated by oral examination. During the semester, each student prepares a lecture on the chosen topic.
Aim of the course -
Last update: Přibyl Michal prof. Ing. Ph.D. (14.11.2018)

Students will be able to:

Formulate mass and heat balances of different types of bioreactors.

Understand the transport and reaction processes taking place in various types of enzyme and cell bioreactors.

Solve mathematical models of bioreactors.

Orient in modern DNA and protein technologies.

Literature -
Last update: Přibyl Michal prof. Ing. Ph.D. (04.05.2022)

Blanch H.W., Clark, D.S., Biochemical Engineering, 2nd edition, Marcel Dekker, 1997, 0-8247-0099-6.

Harrison R.G., Todd P., Rudge S.R., Petrides D.P., Bioseparations science and engineering, 2nd edition, Oxford University Press, 2015, 978-0-19-539181-7.

Villadsen J., Lee S.Y., Nielsen J., Stephanopoulos G. (eds.), Fundamental Bioengineering, Wiley-Blackwell, 2016, 978-3527336746.

Alberts B. et al., Essential Cell Biology, 5th edition, Garland Science, 2019, 978-0-393679533.

Learning resources -
Last update: Přibyl Michal prof. Ing. Ph.D. (14.11.2018)


Syllabus -
Last update: Přibyl Michal prof. Ing. Ph.D. (14.11.2018)

1. Cell types, eukaryotic and prokaryotic cells, cell structure, chemical composition of cells, from DNA to protein, cell cycle, cell energetics, energetics of microbial growth.

2. Enzymes, enzyme reactions, kinetics of enzyme reactions, inhibition of enzymes, effects of pH, ionic strength, temperature.

3. Immobilized enzymes and microbial cells, immobilization methods, effect of immobilization on kinetics of enzyme reactions.

4. Transport in systems with immobilized biocatalysts, limiting process.

5. Kinetics of microbial growth and metabolite production, models of microbial growth.

6. Cell chemotaxis, mathematical model of chemotaxis in spatially one-dimensional system.

7. Types of microbial interactions, mathematical models of microbial interactions, competition and influence of chemotaxis, predator-prey systems.

8. Bioreactors with enzymes and microbial cells, examples of the use, bioreactor control.

9. Mass and heat transfer in bioreactors, transport of oxygen in fermeters, oxygen solubility, kLa.

10. Thermal sterilization, sterilization equipment, heat transfer coefficients.

11. Separation and purification of microbial products, separation of cells from the culture medium, destruction of cells, product separation and purification.

12. Typical sequences of separation steps, economic principles of the downstream process.

13. DNA and protein technology. DNA sequencing, DNA amplification, cDNA libraries, gene engineering.

14. Recombinant proteins, genetically modified organisms (preparation, benefits and risks, examples), preparation of special proteins in genetically engineered

modified organisms, preparation of monoclonal and polyclonal antibodies.

Registration requirements -
Last update: Přibyl Michal prof. Ing. Ph.D. (14.11.2018)

Chemical engineering


Course completion requirements -
Last update: Přibyl Michal prof. Ing. Ph.D. (14.11.2018)

In oral exam, a student answers three questions from a list of questions.