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The subject is focused on understanding of principles of manipulation with nucleic acids and their analysis. It covers information about various methods of introducing genes into diverse cell types, determination of gene expression and analysis. The goal is to deliver information about genetic engineering techniques in order to facilitate the choce of the optimal method for a particular application. The lectures cover basic methods of isolation, analysis and modification of nucleic acids and special applications as gene modification for detection or affinity purification of gene product, study of interaction of proteins and nucleic acids, or gene therapies.
Last update: Kopecká Blanka (29.08.2018)
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Students will be able to:
Isolate DNA and perform basic manipulations with recombinant DNA. Prepare vectors for genetic modification of various cell types.
Modify and label DNA, apply DNA labeled probes for identification of specific sequences.
Modify genes by directed mutagenesis by directed mutagenesis, extension by sequences for detection and purification by affinity chromatography.
Analyze production of heterologous proteins and isolate them from various cell types.
Analyze protein interactions. Construct gene libraries. Last update: Kopecká Blanka (29.08.2018)
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R: Green M. R., Sambrook J., Molecular Cloning: A Laboratory Manual (Fourth Edition); Cold Spring Harbor Laboratory Press 2012 Last update: Ruml Tomáš (31.08.2018)
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1. Introduction genetics 1 2. Introduction genetics 2 3. Introduction genetic engineering 4. Restriction endonucleases 5. Sequence analysis 6. Gene expression 1 7. Gene expression 2 biotechnology 8. PCR 9. DNA sequencing 10. Forensic analysis 11. Protein interactions 12. Gene therapy 1 13. Gene therapy 2 14. Interactions assessment
Last update: Ruml Tomáš (26.09.2018)
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