SubjectsSubjects(version: 916)
Course, academic year 2019/2020
Biochemistry: Laboratory - S320014
Title: Biochemistry: Laboratory
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019 to 2019
Semester: summer
Points: summer s.:4
E-Credits: summer s.:4
Examination process: summer s.:
Hours per week, examination: summer s.:0/6, MC [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
For type:  
Guarantor: Podzimek Tomáš Ing. Ph.D.
Novotná Zuzana doc. Dr. Ing.
Class: Základní laboratoře
Examination dates   Schedule   
Syllabus - Czech
Last update: KNOBLOCL (12.01.2012)

1. Identification of amino acids by paper electrophoresis

2. Identification of proteins by SDS PAGE (polyacrylamide gel electrophoresis)

3. Determination of tripeptide sequence

4. Isolation of DNA from spleen

5. Spectrophotometric determination of protein and DNA concentrations in a mixed sample

6. Separation of macromolecules by gel filtration chromatography

7. Immunoprecipitation reactions in solution

8. Separation of trypsin by ion-exchange chromatography

9. Influence of reaction conditions on trypsin activity

10. Determination of the Michaelis constant using chromogenic substrate

11. Immobilisation of yeasts to alginate gel

12. Titration curves of amino acids