Subjects

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Biochemistry: Seminar I - N320003

Title:	Seminář z biochemie I
Guaranteed by:	Department of Biochemistry and Microbiology (320)
Faculty:	Faculty of Food and Biochemical Technology
Actual:	from 2011 to 2014
Semester:	both
Points:	1
E-Credits:	1
Examination process:
Hours per week, examination:	0/1, MC [HT]
Capacity:	winter:unknown / unlimited (unknown) summer:unknown / unknown (unknown)
Min. number of students:	unlimited
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Teaching methods:	full-time
Level:
Additional information:	http://předmět je vyučován pouze v zimním semestru
Note:	you can enroll for the course in winter and in summer semester

Guarantor:	Benešová Eva Ing. Ph.D. Lipov Jan doc. Ing. Ph.D.
Interchangeability :	Z320003

Examination dates Schedule

Annotation -

Biochemistry: Seminar I is mainly focused on practical knowledge necessary for successful mastery of basic laboratory techniques. It familiarizes students with various separation techniques and immunochemical methods and allows the understanding of their real use in practice using specific examples. In the second half the seminar is focused on natural biocatalysts - enzymes, on their classification, kinetics and inhibition, again using examples from laboratory work.

Last update: Lipov Jan (08.08.2013)

Aim of the course -

Students will be able to:

draw formulas and evaluate charge properties of coded aminoacids

draw the titration curve of peptides

understand basic chromatography techniques (gel permeation, ion exchange, affinity)

understand basics of immunochemical methods

divide enzymes to the classes

understand basics of enzyme kinetics and inhibition

Last update: TAJ320 (26.09.2013)

Literature -

R: Káš J., Kodíček M., Valentová O.: Laboratorní techniky biochemie. 1. vyd. Vysoká škola chemicko-technologická v Praze, Praha 2006. ISBN 80-7080-586-2

A: Nelson D. L., Cox M. M., Principles of Biochemistry, 4. vydání, W.H.Freeman&Co., New York, 2005, 978-0716762652

Last update: Lipov Jan (08.08.2013)

Syllabus -

1. Introduction

2. Aminoacids (structure, classification, peptide formation)

3. Aminoacids (charge properties, isoelectric point)

4. Aminoacids (titration curves, aminoacids as buffers)

5. Separation techniques Ia (gel permeation and ion exchange chromatography)

6. Separation techniques Ib (other chromatochraphy techniques)

7. Separation techniques II - electrophoresis

8. Immunochemical methods

9. Determination of protein concentration

10. Determination of protein primary structure

11. Enzymes (basic properties, classification to classes, nomenclature)

12. Enzyme kinetics (examples)

13. Enzyme inhibition (examples)

14. Final summary and practice

Last update: TAJ320 (26.09.2013)

Learning resources -

Kodíček Milan: Biochemické pojmy : výkladový slovník (http://vydavatelstvi.vscht.cz/knihy/uid_es-002/)

Last update: Lipov Jan (08.08.2013)

Registration requirements -

none

Last update: Benešová Eva (26.08.2013)

Teaching methods
	Activity	Credits	Hours
	Příprava na zkoušku a její absolvování	0.5	14
	Účast na seminářích	0.5	14
		1 / 1	28 / 28

Coursework assessment
Form	Significance
Continuous assessment of study performance and course -credit tests	100