Subjects

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Isolation and Characterisation of Biomacromolecules - N320018

Title:	Izolace a charakterizace biomakromolekul
Guaranteed by:	Department of Biochemistry and Microbiology (320)
Faculty:	Faculty of Food and Biochemical Technology
Actual:	from 2013 to 2018
Semester:	winter
Points:	winter s.:3
E-Credits:	winter s.:3
Examination process:	winter s.:
Hours per week, examination:	winter s.:2/0, Ex [HT]
Capacity:	unlimited / unknown (unknown)
Min. number of students:	unlimited
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Teaching methods:	full-time
Level:
Additional information:	http://biomikro.vscht.cz/vyuka/?Predmet=ib
Note:	course can be enrolled in outside the study plan enabled for web enrollment

Guarantor:	Lipovová Petra doc. Ing. Ph.D. Holubová Barbora doc. Ing. Ph.D.

Examination dates Schedule

Annotation -

The course focuses on understanding the principles of purification techniques for the isolation of biomolecules. The aim is to teach students how to design a proper procedure for the purification of biomolecules target and characterize it. This knowledge can be used in many fields.

Last update: Lipovová Petra (01.07.2013)

Aim of the course -

Students will be able to:

Understand the principles of membrane, chromatographic and electrophoretic techniques.

Propose a procedure to optimize protein purification.

They will know the basic techniques of immobilization of proteins and cells.

Last update: Lipovová Petra (01.07.2013)

Literature -

R: Janson J.C.: Protein Purification, Wiley, New York, 1998, ISBN 0471186260

R: Mikkelsen S.R.: Bioanalytical Chemistry, Wiley, New York, 2004, ISBN 0471544477

Last update: Lipovová Petra (10.07.2013)

Syllabus -

1. Introduction - source of proteins, dezintegration, membrane process

2. Precipitation, extraction, and centrifugation

3. Materials for chromatographic and eletrophoretic methods

4. The basic division and instrumentation

5. Gel-permeation chromatography

6. Ionex chromatography

7. Hydrophobic chromatography and reversed phase chromatography

8. Affinity chromatography

9. Thin layer chromatography, recovery and field of chromatography and determination of proteins concentration

10. Introduction to the electrophoretic methods

11. Types of elektrophoretic methods

12. Immobilisation I

13. Immobilisation II

14. Presentation of project

Last update: Lipovová Petra (01.07.2013)

Learning resources -

http://biomikro.vscht.cz/vyuka/?Predmet=ib

Last update: Lipovová Petra (26.08.2013)

Registration requirements -

Last update: Lipovová Petra (01.07.2013)

Teaching methods
	Activity	Credits	Hours
	Obhajoba individuálního projektu	0.5	14
	Účast na přednáškách	0.5	14
	Práce na individuálním projektu	1	28
	Příprava na zkoušku a její absolvování	1	28
		3 / 3	84 / 84

Coursework assessment
Form	Significance
Regular attendance	10
Report from individual projects	20
Examination test	30
Oral examination	40