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The lectures are focused on separation techniques. Both historically older techniques such as destilation and extraction and modern methods, for example high performance liquid chromatography, capillary electrophoresis, mass spectrometry are presented. Besides well known techniques less common methods that have great separation potencial are also discussed. Laboratory tasks deal with capillary electrophoresis, isotachophoresis, gas and liguid chromatgraphy.
Last update: Řezanka Pavel (25.08.2014)
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Students will be able to:
be well informed about separation methods
solve problems of real samples separations
evaluate and interpret data obtained by separation analysis. Last update: Řezanka Pavel (14.09.2013)
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Předmět je zakončen písemnou a ústní zkouškou. Pro udělení zápočtu student musí student ze zápočtového testu získat alespoň 50% bodů a úspěšně absolvovat laboratoře. Last update: Řezanka Pavel (25.08.2014)
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R: Analytická separace látek - kniha, J. Churáček; SNTL, 1990. R: High Performance Capillary Electrophoresis - firemní příručka, D.N. Heiger, Hewlett-Packard, 1992. R: Basic Gas Chromatography - kniha, H.M. McNair and J.M. Miller, Wiley, 2009, ISBN: 978-0-470-43954-8. R: Practical High-Performance Liquid Chromatography - kniha, V.R. Meyer, Wiley, 2010, ISBN:978-0-470-68218-0. R: Introduction to Mass Spectrometry - kniha, J.T. Watson and O.D. Sparkman, Wiley, 2007, ISBN: 978-0-470-51634-8. A: Mass Spectrometry-A Textbook - kniha, J.H. Gross, 2004, Springer, ISBN: 3-540-40739-1. A: Introduction to Modern Liquid Chromatography - kniha, L.R. Snyder, J.J. Kirkland and J.W. Dolan, Wiley, 2010, ISBN:978-0-470-16754-0. A: Gas Chromatography and Mass Spectrometry-A Practical Guide - kniha, O.D. Sparkman, Z.E. Penton, and F.G. Kitson, 2011, Elsevier, ISBN:978-0-12-373628-4. A: Practical HPLC Method Development - kniha, L.R. Snyder, J.J. Kirkland, J.L. Glajch; Wiley, 1997, ISBN:0-471-00703-X. A: Chromatographic methods - kniha, A. Braithwaite, F.J. Smith; Blackie Academic & Proffessional, 1996, ISBN:0-7514-0158-7. A: Gas Chromatography - kniha, I.A. Fowlis; ACOL, Wiley, 1995, ISBN:0-471-95467-5. A: High Performance Liquid Chromatography - kniha, S. Lindsay; ACOL, Wiley, 1992, ISBN:0-471-93180-2. A: Separace látek - skripta, M. Popl, J. Kubát; SNTL, 1986. A: Chromatography Today - kniha, C.F. Poole, S.K. Poole; Elsevier, 1991, ISBN:0-444-88492-0. Last update: VED402 (01.10.2013)
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Every week there is a two hour lecture and one hour seminar. Last update: Řezanka Pavel (14.09.2013)
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1. The examination is written and takes 60 min.
2. Students, who study in Czech, pass examination in Czech. Students, who study in English, pass Examination in English.
3. Student is duty to present Index with written credit during the examination, otherwise it is impossible to continue in Examination.
4. The students sign in for the examination using SIS and it is duty of student to sign in for the term listed here. Signing off is possible up to date listed in SIS. Later it is possible only by request to secretary of department and only because of serious reasons. The examination is classified "F" when the student is absent and not signed off.
5. The assistant can allow writing examination when student arrives late. The missed time is not compensated.
6. It is not allowed to leave room during examination. It is not allowed to use forbidden materials and tools including telecommunication tools.
7. The place and the time will be announced during the examination. The examiner assign where and when will be possible to see checked examination test. If the student is not present in this time he or she resigns for any complain and agree with results. When student do not agree with classification of any of parts of test, the examiner will provide one, exceptionally more, additional question which will be answered in written form. According to result of these additional questions the examiner can change overall classification of examination by one step up or down.
8. Student can use only two reparative terms. Last update: Řezanka Pavel (14.09.2013)
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1. Introduction to Separation Methods 2. Destilations 3. Extractions 4. Separations Based on Phase Equilibria 5. Membrane Separation 6. Separation in Force Field 7. Electrophoresis 8. Capillary Electrophoresis 9. Capillary Electrochromatography 10. Chromatography 11. Gas Chromatography 12. Liquid Chromatography 13. Supercritical Fluid Chromatography 14. Mass spectrometry Last update: Sýkora David (19.09.2013)
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http://onlinelibrary.wiley.com/book/10.1002/9780470688427 Last update: Řezanka Pavel (14.09.2013)
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The students need to know analytical chemistry on the level of Analytical chemistry I. Last update: Řezanka Pavel (14.09.2013)
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The students need to know analytical chemistry on the level of Analytical chemistry I. Last update: VED402 (24.09.2013)
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| Teaching methods | ||||
| Activity | Credits | Hours | ||
| Konzultace s vyučujícími | 0.5 | 14 | ||
| Účast v laboratořích (na exkurzi nebo praxi) | 1 | 28 | ||
| Účast na přednáškách | 1 | 28 | ||
| Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 1 | 28 | ||
| Příprava na zkoušku a její absolvování | 2 | 56 | ||
| Účast na seminářích | 0.5 | 14 | ||
| 6 / 6 | 168 / 168 | |||
| Coursework assessment | |
| Form | Significance |
| Report from individual projects | 10 |
| Examination test | 60 |
| Continuous assessment of study performance and course -credit tests | 10 |
| Oral examination | 20 |