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Within this course, principles and key applications of modern isolation, separation and detection techniques commonly used in the field of studies / characterization of biologically active compounds. Special attention is paid to (i) application of basic and advanced extraction techniques; (ii) effective separation strategy realized by gas / liquid chromatography; (iii) presentation of various instrumental techniques with a special focus on mass spectrometry and its presentation in case studies. In the final phase of course, practical aspects together with problems to be solved in a qualified manner in practice are summarized. Last update: Dvořáková Darina (22.10.2021)
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To obtain the credit, it is necessary to prepare a group project (3-4 students) in the form of a presentation, which summarizes several case studies in the analysis of a selected group of biologically active substances. The award of credit is a necessary condition for passing the exam, which consists of a written part and possibly an oral exam. Last update: Dvořáková Darina (22.10.2021)
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Stauffer M. (ed.): Calibration and Validation of Analytical Methods, A sampling of Current Approaches (2018), e-source VŠCHT, ISBN: 978-1-78923-085-7; DOI: 10.5772/intechopen.69918
Naushad M. & Khan M.R. (ed): Ultra Performance Liquid Chromatography Mass Spectrometry, Evaluation and Applications in Food Analysis (2014), e-source VŠCHT, ISBN: 9780429073953; https://doi.org/10.1201/b16670
Picó Y. (ed): Advanced Mass Spectrometry for Food Safety and Quality, Comprehensive Analytical Chemistry, Handboook, Volume 68, Pages 3-714 (2015), e-source VŠCHT, ISBN: 978-0-444-63340-8
Kusch P. (ed): Gas Chromatography, Derivatization, Sample Preparation, Application (2019), e-source VŠCHT, ISBN: 978-1-83881-866-1; DOI: 10.5772/intechopen.73339
Mutlu M. (ed): Biosensors in Food Processing, Safety, and Quality Control (2010); e-book: ISBN: 9780429104121; DOI: 10.1201/b10466
Beier R.C. & Stanker L.H. (ed): Immunoassays for Residue Analysis (1996); ISBN13: 9780841233799; DOI: 10.1021/bk-1996-0621 Last update: Dvořáková Darina (09.11.2021)
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Within the extent of the syllabus. Last update: Kubová Petra (16.04.2018)
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1 Introduction to the issue, classification of biologically active substances
2 General characteristics of the analytical procedure, performance characteristics and validation, basic steps in the analysis of biologically active substances of both natural and anthropogenic origin
3 Basic and advanced extraction techniques with a focus on their use in practice
4 Gas chromatography (theory, injection techniques, classification of capillary columns, influence of various parameters on the separation process, optimization of separation, two-dimensional GC, conventional detectors)
5 Liquid chromatography (theory, selection of chromatographic system, columns and classification of stationary phases, conventional detectors, optimization and influence of various parameters on the separation process)
6 Mass spectrometry (introduction and characterization of application potential, ionization techniques, mass analyzers, ion mobility, detection)
7 Analysis of volatile compounds, GC-MS (case studies)
8 Analysis of environmental contaminants by GC-MS (case studies)
9 Analysis of environmental contaminants by LC-MS (case studies)
10 Bioanalytical methods I (biosenzors and portable systems for food analysis)
11 Bioanalytical methods II (principles of biomolecular recognition)
12 Analysis of pesticides and mycotoxins
13 Analysis of vitamins and other important biologically active compounds
14 Presentation of individual students works, discussion, preparation for final test Last update: Dvořáková Darina (22.10.2021)
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Group at MS teams, where students will complete short tests online to verify their knowledge at the end of each teaching block. After the evaluation of the tests, the results will be discussed with the students and the most common mistakes will be explained.
Technique overview:
http://www.ionsource.com/tutorial/spectut/spec1.htm
http://www.chemguide.co.uk/analysis/masspecmenu.htm Last update: Dvořáková Darina (22.10.2021)
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Students will be able to understand:
Last update: Dvořáková Darina (22.10.2021)
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Analytical chemistry, Food chemistry, Analysis of food and natural products Last update: Dvořáková Darina (22.10.2021)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Obhajoba individuálního projektu | 0.5 | 14 | ||
Účast na přednáškách | 1 | 28 | ||
Práce na individuálním projektu | 1 | 28 | ||
Příprava na zkoušku a její absolvování | 1 | 28 | ||
Účast na seminářích | 0.5 | 14 | ||
4 / 4 | 112 / 112 |
Coursework assessment | |
Form | Significance |
Defense of an individual project | 40 |
Examination test | 60 |