Subjects

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Advanced imaging techniques - AP320007

Title:	Advanced imaging techniques
Guaranteed by:	Department of Biochemistry and Microbiology (320)
Faculty:	Faculty of Food and Biochemical Technology
Actual:	from 2019 to 2021
Semester:	both
Points:	0
E-Credits:	0
Examination process:
Hours per week, examination:	3/0, other [HT]
Capacity:	winter:unknown / unknown (unknown) summer:unknown / unknown (unknown)
Min. number of students:	unlimited
State of the course:	taught
Language:	English
Teaching methods:	full-time
Level:
Note:	can be fulfilled in the future you can enroll for the course in winter and in summer semester

Guarantor:	Lipov Jan doc. Ing. Ph.D. Zelenka Jaroslav doc. Ing. Ph.D.
Interchangeability :	P320007

Examination dates Schedule

Annotation -

Microscopy techniques provide an efficient and unique approach to the study of fixed tissues and living cells with high specificity and sensitivity. Course aims to acquaint students with modern microscopy techniques for biology object imaging. Part of the workshop will be the introduction to automated wide-field, confocal and superresolution fluorescent microscopy. First, a brief introduction to the physical mechanisms underlying these imaging techniques will be given. Then we introduce the theoretical and/or practical basis of particular advanced imaging techniques. The basics of image analysis will be covered as well.

Last update: Lipov Jan (02.11.2018)

Course completion requirements -

Active presence of the student on the lectures.

More then 50% from the final test.

Last update: Pátková Vlasta (08.06.2018)

Literature -

Scientific papers and online books from the portfolio of Nature Research, AAAS and others.

Last update: Pátková Vlasta (08.06.2018)

Teaching methods -

Lectures, professional debate, selflearning, excursion.

Last update: Pátková Vlasta (08.06.2018)

Syllabus -

Fluorescence microscopy as a specific part of optical microscopy, quantification of fluorescence, fluorescent tags.

Confocal, multiphoton and correlation microscopy.

The application of fluorescence microscopy - FRAP (Fluorescence Recovery After Photobleaching), FRET (Förster Resonance Energy Transfer)

The application of fluorescence microscopy - TIRF (Total Internal Reflection Fluorescence), SIM (Structured Illumination Microscopy)

The application of fluorescence microscopy - PALM (PhotoActivated Localization Microscopy), STORM (Stochastic Optical Reconstruction Microscopy)

The application of fluorescence microscopy - STED (Stimulated Emission Depletion Microscopy), FLIM (Fluorescence-Lifetime Imaging Microscopy).

Image analysis – parameter ajdustment before acquisition, software for image processing and analysis – deconvolution, 3D visualization, colocalization, tracking, correction of chromatic abberations.

Last update: Lipov Jan (02.11.2018)

Learning outcomes -

Student will be introduced to modern facts and methodology in advanced microscopy of biological objects, she/he will obtain an overview of possible techniques, which allows her/him more qualified decisions in scientific research activities.

Last update: Pátková Vlasta (08.06.2018)

Entry requirements -

Basic knowledge in the field of optics and microscopic techniques.

Last update: Pátková Vlasta (08.06.2018)