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Lab training is designed for works whose result would be few tens of milligrams (ca 50 mg) of pure compound which is a sufficient amount for chemical characterization. The intention is to learn to work with small amounts and with special equipment and labware as is usual for research work isolating compounds of natural origin.
Last update: Kubová Petra (29.01.2018)
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Odevzdání preparátů. Odevzdání laboratorních protokolů ke každé práci. Last update: MORAVCOJ (23.02.2018)
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R: Mayo D. W., Pike R. M., Butcher S. S.: Microscale Oganic Laboratory , John Wiley and Sons, Inc., 2nd ed., New York, 1989 Last update: MORAVCOJ (21.02.2018)
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1. Labor safety in laboratory, construction of apparatuses for basic operations 2. Isolation of caffeine from tea, extraction, sublimation, melting point 3. Isolation of trehalose from yeast, deproteinization, HPLC verification of product purity 4. Isolation of piperine from black pepper, extraction, decantation, TLC, crystallization 5. Isolation of botulin from birch bark, extraction, crystallization, derivatization 6. Isolation of cinnamaldehyde from cinnamon using steam distillation 7. Isolation of D-glucosamine from crab shells, decalcification, hydrolysis, crystallization 8. GLC analysis of cinnamaldehyde 9. HPLC analysis of trehalose on ion-exchange resin 10. NMR spectra measurements of prepared samples 11. IR spectra measurements of prepared samples Last update: Kubová Petra (29.01.2018)
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Instructions for Isolation and Separation methods: Laboratory, available on the web page of the department Last update: MORAVCOJ (21.02.2018)
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Students will be able to: Isolate and purify small amounts of compounds from natural materials Work with labware designed for semi-micro preparative scale Design procedures and equipment suitable for work with small amounts of compounds Perform spectral and other characterization from small amounts of isolated compounds Last update: MORAVCOJ (21.02.2018)
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| Coursework assessment | |
| Form | Significance |
| Regular attendance | 30 |
| Report from individual projects | 70 |