Subjects

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Genetic Engineering - N320051

Title:	Genové inženýrství
Guaranteed by:	Department of Biochemistry and Microbiology (320)
Faculty:	Faculty of Food and Biochemical Technology
Actual:	from 2019 to 2020
Semester:	both
Points:	3
E-Credits:	3
Examination process:
Hours per week, examination:	2/0, Ex [HT]
Capacity:	winter:unknown / unknown (unknown) summer:unknown / unknown (unknown)
Min. number of students:	unlimited
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Level:
Is provided by:	M320017
Note:	you can enroll for the course in winter and in summer semester

Guarantor:	Ruml Tomáš prof. Ing. CSc.
Interchangeability :	S320007
Is interchangeable with:	S320007, M320017, AM320017

Examination dates Schedule

Annotation -

The subject is focused on understanding of principles of manipulation with nucleic acids and their analysis. It covers information about various methods of introducing genes into diverse cell types, determination of gene expression and analysis. The goal is to deliver information about genetic engineering techniques in order to facilitate the choce of the optimal method for a particular application. The lectures cover basic methods of isolation, analysis and modification of nucleic acids and special applications as gene modification for detection or affinity purification of gene product, study of interaction of proteins and nucleic acids, or gene therapies.

Last update: TAJ320 (11.12.2013)

Literature -

R: Ruml T., Rumlová M., Pačes V.: Genové inženýrství; VŠCHT Praha 2002

R: Green M. R., Sambrook J., Molecular Cloning: A Laboratory Manual (Fourth Edition); Cold Spring Harbor Laboratory Press 2012

Last update: TAJ320 (26.09.2013)

Syllabus -

1. Recapitulation of genetic principles.

2. Bacterial plasmid vectors.

3. Basic operations with DNA.

4. Labeling of nucleic acids and application of probes.

5. Analysis of DNA sequences.

6. Principles of work with RNA.

7. Polymerase chain reaction and its applications.

8. Expression in microbial cells, markers, fusion proteins.

9. Tissue cultures and their use for gene expression.

10. Principle of construction of transgenic organisms.

11. Mutations, directed mutagenesis.

12. Genome mapping, restriction analysis.

13. Product detection - metabolic labeling, electrophoretic and immunochemical methods.

14. Methods for study in protein and nucleic acids interactions.

Last update: UST320 (21.08.2013)

Learning resources -

http://biomikro.vscht.cz/vyuka/?Predmet=gieng

Last update: TAJ320 (19.09.2013)

Learning outcomes -

Students will be able to:

Isolate DNA and perform basic manipulations with recombinant DNA. Prepare vectors for genetic modification of various cell types.

Modify and label DNA, apply DNA labeled probes for identification of specific sequences.

Modify genes by directed mutagenesis by directed mutagenesis, extension by sequences for detection and purification by affinity chromatography.

Analyze production of heterologous proteins and isolate them from various cell types.

Analyze protein interactions. Construct gene libraries.

Last update: UST320 (21.08.2013)

Registration requirements -

Biochemistry I

Molecular genetics or Molecular genetics and DNA analysis

Last update: UST320 (21.08.2013)

Teaching methods
	Activity	Credits	Hours
	Konzultace s vyučujícími	0.1	2
	Účast na přednáškách	1	28
	Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi	0.5	14
	Příprava na zkoušku a její absolvování	1.4	40
		3 / 3	84 / 84

Coursework assessment
Form	Significance
Examination test	40
Oral examination	60