SubjectsSubjects(version: 965)
Course, academic year 2019/2020
  
Isolation and Separation Methods - N323023
Title: Izolační a separační metody
Guaranteed by: Department of Food Analysis and Nutrition (323)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019 to 2020
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:2/1, C+Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Level:  
Is provided by: M323010
Additional information: http://web.vscht.cz/poustkaj
Guarantor: Pulkrabová Jana prof. Ing. Ph.D.
Poustka Jan prof. Dr. Ing.
Is interchangeable with: M323010, AM323010
Examination dates   Schedule   
Annotation -
The course is focused on the principles and advanced applications of modern isolation and separation techniques including representative detection methods. Methods are discussed with regard to practical aspects and problems that are normally encountered. Framework of studied methods: isolation in gaseous, liquid, supercritical and solid phase; gas and liquid chromatography, including conventional detectors, mass spectrometry.
Last update: Poustka Jan (03.03.2014)
Literature -

R: Churáček, J. a kol.: Analytická separace látek, SNTL, Praha, ISBN 80-03-00569-8, 1990.

R: Pawliszyn J.: Solid phase microextraction: Theory and Practice. Wiley-VCH, Inc. NY, USA, ISBN 0-471-19034-9, 1997.

R: Dean, J.R.: Extraction Methods for Environmental Analysis, J.Wiley&Sons, Ltd., Chichester, Anglie, ISBN 0-471-98287-3, 1998.

R: Grob, R.L. (Editor): Modern Practice of Gas Chromatography, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-59700-7, 1995.

R: Willoughby, R., Sheehan, E., Mitrovich, S.: A Global View of LC/MS: How to Solve Your Most Challenging Analytical Problems, Global View Publishing, Pittsburg, Pennsylvania, USA, ISBN 0-9660813-0-7, 1998.

A: Snyder,L.R., Kirkland, J.J., Glajch, J.L.: Practical HPLC Method Development, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-00703-X, 1997.

A: Rood, D.: A Practical Guide to the Care Maintenance and Troubleshooting of Capillary Gas Chromatographic Systems, Hüthig GmbH, Heidelberg, Německo, ISBN 3-7785-2319-8, 1995.

A: Scientific papers.

Last update: Poustka Jan (03.03.2014)
Syllabus -

1. General characteristics of analytical procedure and classification of techniques

2. Purge & trap, head space, supercritical fluid extraction

3. Liquid-liquid extraction, liquid micro-extraction

4. Solid-liquid extraction, various means of implementation (ASE-PLE, MASE)

5. Solid phase extraction

6. Solid phase micro-extraction

7. Adsorption chromatography, dispersive solid phase extraction (MSPD, QuEChERS)

8. Gel permeation chromatography

9. Gas chromatography - factors influencing separation, injection techniques

10. Gas chromatography - columns, detectors

11. Gas chromatography - large volume injection; GCxGC

12. Liquid chromatography - factors influencing separation

13. Liquid chromatography - columns, detectors

14. Mass spectrometry

Last update: Poustka Jan (03.03.2014)
Learning resources -

Course outlines at http://web.vscht.cz/poustkaj

Last update: Poustka Jan (03.03.2014)
Learning outcomes -

Students will be able to understand:

1. Principles, technical configurations and application potential of sample preparation methods

2. Principles, technical configurations and application potential of gas and liquid chromatography including the application of conventional detectors

3. Principles, technical configurations and application potential of mass spectrometric methods based on various principles of ion separation

Last update: Poustka Jan (03.03.2014)
Registration requirements -

No requirements

Last update: Poustka Jan (03.03.2014)
Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0.5 14
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0.5 14
Práce na individuálním projektu 0.5 14
Příprava na zkoušku a její absolvování 1 28
Účast na seminářích 0.5 14
4 / 4 112 / 112
Coursework assessment
Form Significance
Report from individual projects 30
Examination test 70

 
VŠCHT Praha