Subjects

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Isolation and Separation Methods - S323023

Title:	Isolation and Separation Methods
Guaranteed by:	Department of Food Analysis and Nutrition (323)
Faculty:	Faculty of Food and Biochemical Technology
Actual:	from 2019 to 2020
Semester:	winter
Points:	winter s.:4
E-Credits:	winter s.:4
Examination process:	winter s.:
Hours per week, examination:	winter s.:2/1, C+Ex [HT]
Capacity:	unknown / unknown (unknown)
Min. number of students:	unlimited
State of the course:	taught
Language:	English
Teaching methods:	full-time
Level:
Is provided by:	AM323010
Additional information:	http://web.vscht.cz/poustkaj

Guarantor:	Pulkrabová Jana prof. Ing. Ph.D. Poustka Jan prof. Dr. Ing.
Is interchangeable with:	AM323010

Examination dates Schedule

Annotation

The course is focused on the principles and advanced applications of modern isolation and separation techniques including representative detection methods. Methods are discussed with regard to practical aspects and problems that are normally encountered. Framework of studied methods: isolation in gaseous, liquid, supercritical and solid phase; gas and liquid chromatography, including conventional detectors, mass spectrometry.

Last update: HOLADOVK (16.07.2013)

Literature

Z: Pawliszyn J.: Solid phase microextraction: Theory and Practice. Wiley-VCH, Inc. NY, USA, ISBN 0-471-19034-9, 1997

Z: Dean, J.R. : Extraction Methods for Environmental Analysis, J.Wiley&Sons, Ltd., Chichester, Anglie, ISBN 0-471-98287-3, 1998

Z: Grob, R.L. (Editor) : Modern Practice of Gas Chromatography, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-59700-7, 1995

Z: Willoughby, R., Sheehan, E., Mitrovich, S.: A Global View of LC/MS: How to Solve Your Most Challenging Analytical Problems, Global View Publishing, Pittsburg, Pennsylvania, USA, ISBN 0-9660813-0-7, 1998

D: Snyder,L.R., Kirkland, J.J., Glajch, J.L.: Practical HPLC Method Development, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-00703-X, 1997

D: Rood, D.: A Practical Guide to the Care Maintenance and Troubleshooting of Capillary Gas Chromatographic Systems, Hüthig GmbH, Heidelberg, Německo, ISBN 3-7785-2319-8, 1995

D: Scientific papers

Last update: HOLADOVK (16.07.2013)

Syllabus

1. General characteristics of analytical procedure and classification of techniques

2. Purge & trap, head space, supercritical fluid extraction

3. Liquid-liquid extraction, liquid micro-extraction

4. Solid-liquid extraction, various means of implementation (ASE-PLE, MASE)

5. Solid phase extraction

6. Solid phase micro-extraction

7. Adsorption chromatography, dispersive solid phase extraction (MSPD, QuEChERS)

8. Gel permeation chromatography

9. Gas chromatography - factors influencing separation, injection techniques

10. Gas chromatography - columns, detectors

11. Gas chromatography - large volume injection; GC x GC

12. Liquid chromatography - factors influencing separation

13. Liquid chromatography - columns, detectors

14. Mass spectrometry

Last update: HOLADOVK (07.06.2013)

Learning resources

Course outlines at http://web.vscht.cz/poustkaj

Last update: Poustka Jan (28.06.2013)