Subjects

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Molecular genetics and DNA analysis - N320081

Title:	Molekulová genetika a analýza DNA
Guaranteed by:	Department of Biochemistry and Microbiology (320)
Faculty:	Faculty of Food and Biochemical Technology
Actual:	from 2020 to 2020
Semester:	winter
Points:	winter s.:3
E-Credits:	winter s.:3
Examination process:	winter s.:
Hours per week, examination:	winter s.:2/0, Ex [HT]
Capacity:	unlimited / unknown (unknown)
Min. number of students:	unlimited
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Teaching methods:	full-time
Level:

Guarantor:	Ruml Tomáš prof. Ing. CSc.
Is interchangeable with:	B320015, AB320015

Examination dates Schedule

Annotation -

The subject is focused on understanding heredity principles especially mechanisms and regulation of transfer of genetic information and gene expression in prokaryotic and eukaryotic organisms. Special emphasis will be put on the main regulatory processes mediated by proteins and RNA regulatory molecules. Other focus is on understanding the variability of genetic information due to mutations and recombination. The subject offers information on the most important methods of DNA analysis as DNA identification, sequencing and analysis of selected sequences by using polymerase chain reaction.

Last update: Ruml Tomáš (13.09.2019)

Aim of the course -

Students will know:

Basic principles of molecular genetics and the relationship of their structure and function.

Principles of regulation of transfer of genetic information and its variability due to mutations and recombination

Mechanisms involved in mutation repair.

Basic processes of gene expression as regulation of transcription, in prokaryotes and eukaryotes and posttranscriptional modifications of eukaryotic mRNA, regulation of stability of mRNA and translation, role of small RNAs.

Basic principles of work with DNA, methods of DNA analysis and basic applications of polymerase chain reaction

Last update: TAJ320 (19.09.2013)

Literature -

Michael J Simmons, Peter D. Snustad: Genetika, Nakladatel: Masarykova univerzita Brno 2017

Lodish, H.; et al. Molecular cell biology, 8th ed.;W.H.Freeman & Co Ltd, 2016.

Albrets B; et al. Molecular biology of the cell, 5th ed. Garland Science, 2008.

Clark D.P. Molecular biology, Elsevier Inc., 2005.

Hitler J.M. Forensic DNA Typing, 2nd ed. Elsevier 2005.

Last update: Ruml Tomáš (13.09.2019)

Syllabus -

1. Historical overview, basic genetic concepts and laws

2. Structure and function of nucleic acids, genome organization

3. Replication I, general features, prokaryotes

4. Replication II, eukaryotes, replisome complex

5. Recombination I, gene segregation in meiosis

6. Recombination II, transposition

7. Mutation and repair mechanisms

8. Methods of DNA sequence analysis, genome mapping

9. Regulation of transcription

10. Posttranscriptional modification of RNA

11. Translation I, initiation, elongation and termination, differences between prokaryotes and eukaryotes

12. Translation II, posttranslational modifications

13. Basic principles of pharmacogenomics

14. Basics of oncogenesis

Last update: Ruml Tomáš (13.09.2019)

Learning resources -

none

Last update: TAJ320 (26.09.2013)

Entry requirements -

To enroll in the course it is required enrollment in courses N110004 A N320004

Last update: TAJ320 (19.09.2013)

Registration requirements -

Biochemistry I

Last update: TAJ320 (26.09.2013)

Teaching methods
	Activity	Credits	Hours
	Konzultace s vyučujícími	0	1
	Účast na přednáškách	1	28
	Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi	0.8	21
	Příprava na zkoušku a její absolvování	1.1	31
		3 / 3	81 / 84

Coursework assessment
Form	Significance
Examination test	40
Oral examination	60