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The subject "Separation Methods in Biotechnology" expands students knowledge oriented towards engineering and technical aspects of biotechnological processes. Students will become familiar with separation processes as unit operations, their principles, roles and inclusion into biotechnologies.
Last update: Melzoch Karel (09.04.2025)
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Participation in the lectures is voluntary. Submission of a protocol from independent work on the project and its oral defense is a prerequisite for obtaining credit. Consultations are provided on request.
Last update: Halecký Martin (15.05.2025)
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Teaching is carried out in the form of lectures. Exercises are carried out in the form of independent individual work on a project with the end of the presentation and defense of the project. Last update: Halecký Martin (15.05.2025)
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As part of the credit, students will prepare a short project on the assigned topic in the range of 3 pages of standardized text (MS Word), prepare a presentation (MS PowerPoint) and present this project to other students in one of the last classes. The exam is oral, the condition of registration for the exam is obtaining credit. Last update: Halecký Martin (15.05.2025)
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1. Biotechnological process as sequence of unit operations - from substrate to product 2. Selection of useful separation process - properties of separated materials and bioproducts 3. Separation processes based on different particle size. Membrane processes, membranes, modules - basic characteristics 4. Filtration, microfiltration, ultrafiltration, nanofiltration, reverse osmosis 5. Membrane distillation, pervaporation, gas permeation 6. Electromigration processes - electrophoresis 7. Elektrodialysis, membrane electrolysis 8. Liquid membranes, pertraction, reactive membranes 9. Supercritical extraction. Two-phase water extraction. Extraction of biopolymers 10. Preparative chromatography - principles, stationary phases and arrangement. Adsorption chromatography. Molecular sieve chromatography 11. Ion-exchange chromatography 12. Hydrophobic interaction chromatography, reverse phase chromatography, affinity chromatography - biospecific interactions 13. Integrated processes. Membrane bioreactors. Industrial applications. 14. How to choose effective separation and purification sequence - strategy and economy Last update: Melzoch Karel (09.04.2025)
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http://eso.vscht.cz http://knihovna.vscht.cz/?q=e-knihy (e-books available in the domain of ICT Prague) Last update: Hladíková Jana (20.12.2017)
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Students will be able to: Define the structure of biotechnological process Undersand the bioprocesses from engineering perspective Describe the separation processes as a unit operation Describe the function and role separation processes in biotechnology Define the fundamental principles of separation processes Integrate the separation processes into biotechnology Select suitable separation process with regard to the requirements for quality and purity of the product Choose the sequence of separation processes, particularly in the isolation, purification and finishing of the product Optimize the separation processes in the up-stream and down-stream processing Clasify separation techniques in terms of efficiency and economy
As part of the exercise, students will prepare and present a case study on a topic from the area of separation processes, for which they will receive credit. Last update: Melzoch Karel (17.09.2023)
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Basic knowledge of physical chemistry, chemical engineering, biochemistry, microbiology, bioengineering, food and biochemical technologies. Last update: Halecký Martin (15.05.2025)
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Basic knowledge of physical chemistry, chemical engineering, biochemistry, microbiology, bioengineering, food and biochemical technologies. Last update: Halecký Martin (15.05.2025)
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| Teaching methods | ||||
| Activity | Credits | Hours | ||
| Obhajoba individuálního projektu | 0.1 | 4 | ||
| Účast na přednáškách | 1 | 28 | ||
| Práce na individuálním projektu | 0.5 | 14 | ||
| Příprava na zkoušku a její absolvování | 1 | 28 | ||
| Účast na seminářích | 0.5 | 14 | ||
| 3 / 4 | 88 / 112 | |||
| Coursework assessment | |
| Form | Significance |
| Defense of an individual project | 15 |
| Report from individual projects | 15 |
| Oral examination | 70 |

