SubjectsSubjects(version: 982)
Course, academic year 2026/2027
  
   
Separation Methods in Biotechnology - M319006
Title: Separace v biotechnologiích
Guaranteed by: Department of Biotechnology (319)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2026
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:2/1, C+Ex [HT]
Capacity: unknown / unknown (unknown)Schedule is not published yet, this information might be misleading.
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Level:  
Additional information: https://e-learning.vscht.cz/course/view.php?id=456
Guarantor: Halecký Martin doc. Ing. Ph.D.
Melzoch Karel prof. Ing. CSc.
Interchangeability : N319009
Examination dates   Schedule   
This subject contains the following additional online materials
Annotation -
The subject "Separation Methods in Biotechnology" expands students knowledge oriented towards engineering and technical aspects of biotechnological processes. Students will become familiar with separation processes as unit operations, their principles, roles and inclusion into biotechnologies.
Last update: Melzoch Karel (09.04.2025)
Course completion requirements -

Participation in the lectures is voluntary. Submission of a protocol from independent work on the project and its oral defense is a prerequisite for obtaining credit.

Consultations are provided on request.

Last update: Halecký Martin (15.05.2025)
Literature -

Obligatory:

  • Kadlec, Pavel, Melzoch, Karel, Voldřich, Michal. Procesy a zařízení potravinářských a biotechnologických výrob. Praha: VŠCHT, 2012, 494 s. ISBN 978-80-7418-086-6 .
  • Wang, William K.. Membrane separations in biotechnology. New York: Marcel Dekker, 2001, 400 s. ISBN 0-8247-0248-4.
  • Fellows, Peter. Food processing technology, principles and practice. Boca Raton, Cambridge: Woodhead Publishing, 2017, 913 s. ISBN 978-0-08-101907-8.

Recommended:

  • Šípek, Milan. Základní membránové procesy. Praha: VŠCHT Praha, 2018, 62 s. ISBN 978-80-7592-023-2.
  • Harrison, Roger G., Todd, Paul, Rudge, Scott R., Petrides, Demetri P.. Bioseparations science and engineering. Oxford: Oxford University Press, 2015, 547 s. ISBN 978-0-19-539181-7.

Last update: Halecký Martin (15.05.2025)
Teaching methods -

Teaching is carried out in the form of lectures. Exercises are carried out in the form of independent individual work on a project with the end of the presentation and defense of the project.

Last update: Halecký Martin (15.05.2025)
Requirements to the exam -

As part of the credit, students will prepare a short project on the assigned topic in the range of 3 pages of standardized text (MS Word), prepare a presentation (MS PowerPoint) and present this project to other students in one of the last classes.

The exam is oral, the condition of registration for the exam is obtaining credit.

Last update: Halecký Martin (15.05.2025)
Syllabus -

1. Biotechnological process as sequence of unit operations - from substrate to product

2. Selection of useful separation process - properties of separated materials and bioproducts

3. Separation processes based on different particle size. Membrane processes, membranes, modules - basic characteristics

4. Filtration, microfiltration, ultrafiltration, nanofiltration, reverse osmosis

5. Membrane distillation, pervaporation, gas permeation

6. Electromigration processes - electrophoresis

7. Elektrodialysis, membrane electrolysis

8. Liquid membranes, pertraction, reactive membranes

9. Supercritical extraction. Two-phase water extraction. Extraction of biopolymers

10. Preparative chromatography - principles, stationary phases and arrangement. Adsorption chromatography. Molecular sieve chromatography

11. Ion-exchange chromatography

12. Hydrophobic interaction chromatography, reverse phase chromatography, affinity chromatography - biospecific interactions

13. Integrated processes. Membrane bioreactors. Industrial applications.

14. How to choose effective separation and purification sequence - strategy and economy

Last update: Melzoch Karel (09.04.2025)
Learning resources -

http://eso.vscht.cz

http://knihovna.vscht.cz/?q=e-knihy (e-books available in the domain of ICT Prague)

Last update: Hladíková Jana (20.12.2017)
Learning outcomes -

Students will be able to:

Define the structure of biotechnological process

Undersand the bioprocesses from engineering perspective

Describe the separation processes as a unit operation

Describe the function and role separation processes in biotechnology

Define the fundamental principles of separation processes

Integrate the separation processes into biotechnology

Select suitable separation process with regard to the requirements for quality and purity of the product

Choose the sequence of separation processes, particularly in the isolation, purification and finishing of the product

Optimize the separation processes in the up-stream and down-stream processing

Clasify separation techniques in terms of efficiency and economy

As part of the exercise, students will prepare and present a case study on a topic from the area of separation processes, for which they will receive credit.

Last update: Melzoch Karel (17.09.2023)
Entry requirements -

Basic knowledge of physical chemistry, chemical engineering, biochemistry, microbiology, bioengineering, food and biochemical technologies.

Last update: Halecký Martin (15.05.2025)
Registration requirements -

Basic knowledge of physical chemistry, chemical engineering, biochemistry, microbiology, bioengineering, food and biochemical technologies.

Last update: Halecký Martin (15.05.2025)
Teaching methods
Activity Credits Hours
Obhajoba individuálního projektu 0.1 4
Účast na přednáškách 1 28
Práce na individuálním projektu 0.5 14
Příprava na zkoušku a její absolvování 1 28
Účast na seminářích 0.5 14
3 / 4 88 / 112
Coursework assessment
Form Significance
Defense of an individual project 15
Report from individual projects 15
Oral examination 70

 
VŠCHT Praha