SubjectsSubjects(version: 990)
Course, academic year 2026/2027
  
   
Cultivation Techniques - M319022
Title: Kultivační techniky
Form of teaching: lecture+laboratory practicals
Guaranteed by: Department of Biotechnology (319)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2026
Duration in semesters: 1
Semester: winter
Points: winter s.:7
E-Credits: winter s.:7
Examination process: winter s.:
Hours per week, examination: winter s.:1/7, MC [HT]
Capacity: unlimited / unlimited (unknown)
Maximum number of enrolled students: unlimited
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Level:  
Repeated enrollment: - / - / - / 9
Guarantor: Křížová Ivana Ing. Ph.D.
Class: Základní laboratoře
Interchangeability : N319040
Examination dates   Schedule   
This subject contains the following additional online materials
Annotation -
The course aims to develop a comprehensive understanding of the design and preparation of a biotechnological experiment, its execution, data analysis, and interpretation of the relationship between experimental conditions and the resulting data. Students will gain the skills necessary to independently carry out basic biotechnological techniques, conduct subsequent analyses, and competently design the entire process, including the final interpretation of findings
Last update: Branská Barbora (25.04.2025)
Course completion requirements -

Participation in practical lessons

Elaboration of reports from individual tasks

Last update: Křížová Ivana (21.07.2026)
Literature -

Obligatory:

Last update: Branská Barbora (25.04.2025)
Teaching methods -

Students work individually or in groups in the laboratory under the supervision of an expert instructor, carrying out assigned tasks. For specific assignments, they collect original data, which they then independently process and present in the form of reports.

Last update: Křížová Ivana (21.07.2026)
Requirements to the exam -

Attendance at laboratory sessions is mandatory (each laboratory block begins with an entry quiz to assess students’ preparedness).

Successful course completion also requires submission of lab reports for the completed assignments.

Last update: Křížová Ivana (21.07.2026)
Syllabus -

1. Introduction to Laboratory Safety, Data Management, and Laboratory Documentation

2. Preparation of Culture Media and Maintenance of Production Microbial Strains

3. Methods for Assessing Cell Growth

4. Molecular Biology Techniques and Their Applications in Biotechnology

5. Production, Isolation, and Analysis of Recombinant Proteins

6. Applications of Fluorescence Microscopy and Flow Cytometry for the Assessment of Microbial Viability

Last update: Křížová Ivana (21.07.2026)
Learning outcomes -

Upon successful completion of the course, students will be able to perform laboratory experiments according to established protocols, apply fundamental biochemical laboratory techniques, adhere to laboratory safety regulations, analyse and interpret experimental data, and document their findings in laboratory reports.

Last update: Křížová Ivana (21.07.2026)
Entry requirements -

Bioengineering, Microbiology

Last update: Branská Barbora (25.04.2025)
Registration requirements -

Basic knowledge of bioengineering and microbiology is assumed.

Last update: Branská Barbora (14.05.2025)
Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0.7 20
Účast v laboratořích (na exkurzi nebo praxi) 2 56
Obhajoba individuálního projektu 0.2 6
Účast na přednáškách 0.5 14
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1.3 36
Práce na individuálním projektu 1.3 36
Účast na seminářích 1 28
7 / 7 196 / 196
Coursework assessment
Form Significance
Defense of an individual project 20
Report from individual projects 80

 
VŠCHT Praha