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Principles of molecular genetics diagnostics of patients with monogenic disorder. Students will learn methods of Sanger and massive parallel sequencing.
Last update: Pátková Vlasta (12.02.2021)
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Students will be able to isolate DNA and prepare a PCR product for Sanger sequencing. They will be able to explain Sanger sequencing reaction and to prepare a library for massive parallel sequencing. Students will be able to analyze the results of sequencing. Last update: Pátková Vlasta (12.02.2021)
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Klasifikovaný zápočet
Last update: Pátková Vlasta (15.02.2021)
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HISTOLOGICKÁ TECHNIKA - M. Jirkovská, Praha, Galén 2006, 2010 Junquierovy základy histologie - A.L.Mescher, 14.vydání, 1. české, Praha, Galén, 2018 Last update: Pátková Vlasta (12.02.2021)
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Introduction to safety instructions Introduction – principles of methods Computer work – sequence retrieval, gene structure, primer localization, PCR product length Laboratory work – DNA isolation Laboratory work – DNA concentration measurement Laboratory work – PCR Laboratory work – Agarose electrophoresis – quality control of the PCR product Laboratory theoretical work – Sequencing reaction preparation Computer work – Sanger and massive parallel sequencing results analysis including its clinical interpretation General histology – microscopic structure of tissues Special histology – microscopic structure of organs Sampling, fixation and analysis of materials for histology laboratory techniques Laboratory practicum – microtom and cryostat techniques Basic and special staining methods Laboratory practicum – staining of tissue slides by hematoxylin and eosin method Principles of histochemistry and immunohistochemistry Laboratory practicum – detection of polysaccharides and lipids Laboratory practicum – immunohistochemical detection Basic microscopy, overview of microscopic methods Laboratory practicum – microscopy of slides prepared during the practicum Laboratory practicum – demonstration of confocal microscope and the digital microscopy laboratory
Last update: Pátková Vlasta (12.02.2021)
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