SubjectsSubjects(version: 948)
Course, academic year 2023/2024
  
Isolation and Separation Methods - M323010
Title: Izolační a separační metody
Guaranteed by: Department of Food Analysis and Nutrition (323)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2023
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:3/0, Ex [HT]
Capacity: unlimited / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Level:  
For type: Master's (post-Bachelor)
Additional information: https://e-learning.vscht.cz/
Guarantor: Poustka Jan prof. Dr. Ing.
Hrbek Vojtěch doc. Ing. Ph.D.
Interchangeability : AM323010, N323023
Is interchangeable with: AM323010
This subject contains the following additional online materials
Annotation -
Last update: Poustka Jan prof. Dr. Ing. (19.01.2018)
The course is focused on the principles and advanced applications of modern isolation and separation techniques including representative detection methods. Methods are discussed with regard to practical aspects and problems that are normally encountered. Framework of studied methods: isolation methods in gaseous, liquid, supercritical and solid phase; gas and liquid chromatography, including conventional detectors; mass spectrometry.
Aim of the course -
Last update: Fialová Jana (19.12.2017)

Students will be able to understand:

1. Principles, technical configurations and application potential of sample preparation methods

2. Principles, technical configurations and application potential of gas and liquid chromatography including the application of conventional detectors

3. Principles, technical configurations and application potential of mass spectrometric methods based on various principles of ion separation

Literature -
Last update: Poustka Jan prof. Dr. Ing. (13.09.2023)

Pawliszyn J.: Solid phase microextraction: Theory and Practice. Wiley-VCH, Inc. NY, USA, ISBN 0-471-19034-9, 1997

Dean, J.R.: Extraction Methods for Environmental Analysis, J.Wiley&Sons, Ltd., Chichester, Anglie, ISBN 0-471-98287-3, 1998

Grob, R.L. (Editor): Modern Practice of Gas Chromatography, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-59700-7, 1995

Willoughby, R., Sheehan, E., Mitrovich, S.: A Global View of LC/MS: How to Solve Your Most Challenging Analytical Problems, Global View Publishing, Pittsburg, Pennsylvania, USA, ISBN 0-9660813-0-7, 1998

Snyder,L.R., Kirkland, J.J., Glajch, J.L.: Practical HPLC Method Development, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-00703-X, 1997

Scientific papers

Learning resources -
Last update: Poustka Jan prof. Dr. Ing. (13.09.2023)

https://e-learning.vscht.cz/

Requirements to the exam -
Last update: Vlčková Martina Ing. (30.01.2018)

Within the extent of the syllabus.

Syllabus -
Last update: Fialová Jana (19.12.2017)

1. General characteristics of analytical procedure and classification of techniques

2. Purge & trap, head space, supercritical fluid extraction

3. Liquid-liquid extraction, liquid micro-extraction

4. Solid-liquid extraction, various means of implementation (ASE-PLE, MASE)

5. Solid phase extraction

6. Solid phase micro-extraction

7. Adsorption chromatography, dispersive solid phase extraction (MSPD, QuEChERS)

8. Gel permeation chromatography

9. Gas chromatography - factors influencing separation, injection techniques

10. Gas chromatography - columns, detectors

11. Gas chromatography - large volume injection; GCxGC

12. Liquid chromatography - factors influencing separation

13. Liquid chromatography - columns, detectors

14. Mass spectrometry

Registration requirements -
Last update: Fialová Jana (19.12.2017)

No requirements

Course completion requirements -
Last update: Poustka Jan prof. Dr. Ing. (19.01.2018)

Successful completion of a written examination.

Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0.5 14
Účast na přednáškách 1.5 42
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0.5 14
Příprava na zkoušku a její absolvování 1.5 42
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