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Last update: Lipov Jan doc. Ing. Ph.D. (08.08.2013)
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Last update: Jablonská Eva Ing. Ph.D. (06.09.2019)
Students will be able to: understand the basics of DNA and RNA work isolate DNA from common sources understand the basics of nucleic acid analysis by restriction cleavage (RFLP) understand the basics of quantification of DNA using real-time PCR understand the basics of human identification using VNTR and STR polymorphism understand the basics of DNA sequencing and fragment analysis |
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Last update: Lipov Jan doc. Ing. Ph.D. (08.08.2013)
A: Butler J.M., Fundamentals of Forensic DNA Typing, Elsevier Publishing 2009, 978-0123749994 |
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Last update: Lipov Jan doc. Ing. Ph.D. (30.08.2013)
none |
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Last update: Jablonská Eva Ing. Ph.D. (06.09.2019)
1. Introduction, safety in molecular biology laboratory 2. Basics of work with biological material, namely DNA 3. Isolation of DNA from buccal swab, quality and quantity assessment 4. Polymerase chain reaction (PCR) - amplification of tandem repeats (locus D1S80) 5. Electrophoretic separation of amplicons 6. Determination of number of repeats (locus D1S80) and random match probability (RMP) 7. Amplification of mitochondrial DNA (mtDNA) using PCR 8. RFLP analysis of mtDNA - cleavage of DNA by restriction endonucleases 9. Electrophoretic separation and analysis of rectriction fragments 10. Nucleic acid quantification using quantitative PCR (qPCR) 11. Multiplex PCR of short tandem repeats (STR) 12. Fragment analysis of amplicons using capillary electrophoresis 13. Evaluation of STR profiles, determination of RMP, analysis of mixed profiles 14. Basics of paternity evaluation based on STR |
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Last update: Lipov Jan doc. Ing. Ph.D. (30.08.2013)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Účast v laboratořích (na exkurzi nebo praxi) | 1 | 28 | ||
Příprava na zkoušku a její absolvování | 1 | 28 | ||
2 / 2 | 56 / 56 |
Coursework assessment | |
Form | Significance |
Report from individual projects | 50 |
Examination test | 50 |