Studenti budou umět:

• Základní principy kapalinové chromatografie

• Optimalizovat podmínky separace a detekce

• Využít techniku HPLC pro stanovení přirozených a kontaminujících složek potravin

Students will be able to:

• Understand the basic principles of liquid chromatography

• Optimize the separation and detection conditions

• Applied HPLC technique for detection of natural food components and contaminants

složení písemné zkoušky

prezentace a obhajoba vlastního projektu

written examination

project presentation and discussion

Knihy

Food Analysis by HPLC – Nollet (2000)

Practical HPLC – Meyer (1996)

Practical HPLC Method development – L.R.Snyder, J.J. Kirkland, J. L. Glajch (1997)

LC/MS a practical users guide – M. C. McMaster (2005)

Pitfalls and Errors of HPLC in Pictures - V. R. Meyer (2006)

elektronická kniha:

HPLC and CE: Principles and Practice

Andrea Weston and Phyllis R. Brown (1997)

http://www.sciencedirect.com/science/book/9780121366407

Časopisy

Journal of Chromatography

Chromatographia

LC-GC International

Journal of Liquid Chromatography

www

LC Troubleshooting: http://www.lcgceurope.com/lcgceurope/article/articleList.jsp?categoryId=535

HPLC guide

http://www.separationsnow.com/coi/cda/home.cda;jsessionid=4EAB9422438DB4C4A206CBBE16FD089A?chId=4

High Performance Liquid Chromatography (HPLC): A Users Guide http://kerouac.pharm.uky.edu/ASRG/HPLC/hplcmytry.html

http://kerouac.pharm.uky.edu/asrg/hplc/applications.html

Practical HPLC Method Development, 2nd Edition (Hardcover)

http://www.amazon.com/exec/obidos/ASIN/047100703X/entertmusicvi-20/103-0437811-9739029

Links to Various Resources, topics, courses

http://www.forumsci.co.il/HPLC/links.html

HPLC Chromatography Books

http://www.chromtech.net.au/amazon-hplc-books1.cfm

Basic Liquid Chromatography

http://hplc.chem.shu.edu/NEW/HPLC_Book/

HPLC portal

http://www.hplcweb.com/

The HPLC troubleshooter

http://www.dq.fct.unl.pt/QOF/hplcts.html

useful HPLC links

http://www.lcresources.com/resources/reslinks.html

e-learning HPLC

http://www.academysavant.com/elearning/elearnHPLC.htm

Books

Food Analysis by HPLC - Nollet (2000)

Practical HPLC - Meyer (1996)

Practical HPLC Method development - L.R.Snyder, J.J. Kirkland, J. L. Glajch (1997)

LC/MS a practical users guide - M. C. McMaster (2005)

Pitfalls and Errors of HPLC in Pictures - V. R. Meyer (2006)

electronic book:

HPLC and CE: Principles and Practice

Andrea Weston and Phyllis R. Brown (1997)

http://www.sciencedirect.com/science/book/9780121366407

Journals:

Journal of Chromatography

Chromatographia

LC-GC International

Journal of Liquid Chromatography

www

LC Troubleshooting: http://www.lcgceurope.com/lcgceurope/article/articleList.jsp?categoryId=535

HPLC guide

http://www.separationsnow.com/coi/cda/home.cda;jsessionid=4EAB9422438DB4C4A206CBBE16FD089A?chId=4

High Performance Liquid Chromatography (HPLC): A Users Guide http://kerouac.pharm.uky.edu/ASRG/HPLC/hplcmytry.html

http://kerouac.pharm.uky.edu/asrg/hplc/applications.html

Practical HPLC Method Development, 2nd Edition (Hardcover)

http://www.amazon.com/exec/obidos/ASIN/047100703X/entertmusicvi-20/103-0437811-9739029

Links to Various Resources, topics, courses

http://www.forumsci.co.il/HPLC/links.html

HPLC Chromatography Books

http://www.chromtech.net.au/amazon-hplc-books1.cfm

Basic Liquid Chromatography

http://hplc.chem.shu.edu/NEW/HPLC_Book/

HPLC portal

http://www.hplcweb.com/

The HPLC troubleshooter

http://www.dq.fct.unl.pt/QOF/hplcts.html

useful HPLC links

http://www.lcresources.com/resources/reslinks.html

e-learning HPLC

http://www.academysavant.com/elearning/elearnHPLC

1. Úvod, princip separace, základní vztahy

2. Nové trendy

3. Mobilní fáze

4. Vývoj metody - volba a optimalizace chromatografického separačního postupu

5. Faktory ovlivňující HPLC stanovení

6. Problémy v HPLC a jejich řešení

7. Detekce - konvenční detektory

8. Detekce – hmotnostní detektory

9. Derivatizační techniky, injekční techniky

10. Aplikace HPLC v analýze potravin

11. Aplikace LC/MS v analýze potravin

12. Případové studie, jejich prezentace

13. Případové studie, jejich prezentace

14. Závěrečný test, zkoušení

1 Introduction, principles of separation, main principles

2 New trends

3 Mobile phases

4 Methods development - optimization of the chromatographic separation process

5 Factors affecting the HPLC determination

6 Problems in HPLC and their solutions

7 Detection - conventional detectors

8 Detection - mass detectors

9 Derivatization techniques, injection techniques

10 Application of HPLC in food analysis

11 Application of LC / MS analysis of food

12 Case studies and their presentation

13 Case studies and their presentation

14 The final test, examination

http://web.vscht.cz/schulzov

http://web.vscht.cz/schulzov

Analytická chemie

Analýza potravin

Analytical Chemistry

Food Analysis