Students will be able to:

Choose suitable analytical method with appropriate detection limit.

Apply chosen method for samples of different origin.

Orientate themselves in specialized literature concerning biomolecules issue.

Obtain wide knowledge of bioanalytical methods spectra including the possibility and limits of their application.

Z: Králová B., Rauch P., Fukal L., Bioanalytická chemie, Vydavatelství VŠCHT Praha, 2007, ISBN 80-7080-449-1

Z: Káš J., Kodíček M., Valentová O., Laboratorní techniky biochemie, Praha 2005, ISBN 80-7080-586-2

Z: Ruml T., Rumlová M., Pačes V., Genové inženýrství, Praha 2002, ISBN 80-7080-499-8

Additional list of suggested bibliography and electronic references will be provided within individual lectures.

. Bioanalytical methods, practical applications, clinical analysis, medicinal chemistry.

2. Basics of combinatorial chemistry, chemical libraries analysis.

3. Principles of biopolymer analysis (DNA, proteins, polysacharides).

4. Biosensors, construction, applications.

5. Immuno assay methods (ELISA, RIA).

6. Enzymology. Systematic classification of enzymes, enzyme kinetics. Immobilized enzymes.

7. Determination of substrates and metabolites of enzyme reactions.

8. Experimental techniques of enzyme methods. Application in clinic analyses and food industry.

9. Analysis of important categories of biologically important compounds.

10. Chromatography of biomolecules. HPLC, FPLC.

11. Afinity chromatography (incl. HAPLC), methods based on molecular recognition.

12. Electroforetic methods and their importance for analytics of biologically important compounds.

13. Usage of modern analytical methods for biopolymers (NMR, X-ray, MS).

14. Analytical methods in medicinal chemistry for new drug development.

Analytical chemistry I

Biochemistry I

Oral examination