SubjectsSubjects(version: 912)
Course, academic year 2022/2023
Bioanalytical Methods - AB320006
Title: Bioanalytical Methods
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2021
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
For type: Bachelor's
Guarantor: Svoboda Petr Ing. Ph.D.
Interchangeability : N320006, N320006A
Annotation -
Last update: Kubová Petra Ing. (19.08.2019)
Bioanalytical methods are applied to determination of analytes using specific interactions between molecules. Modern bioanalytical techniques provide high specificity of analysis (one of reagents is biopolymer and that is why we talk about biospecificity), but also high sensitivity and low detection limit. Part of lectures will cover enzymatic methods, proteomics, electromigration methods, genetic methods (particularly PCR), immunochemical and other biochemical and microbiological methods.
Aim of the course -
Last update: Kubová Petra Ing. (19.08.2019)

Students will be able to:

Choose suitable analytical method with appropriate detection limit.

Apply the chosen method for samples of different origin.

Orientate themselves in specialized literature concerning biomolecules issue.

Obtain wide knowledge of bioanalytical methods spectra including possibility and limits of their application.

Literature -
Last update: Kubová Petra Ing. (19.08.2019)

R: Králová B., Rauch P., Bioanalytické metody, Vydavatelství VŠCHT Praha, 2. vydání 1995, ISBN 80-7080-449-1

R: Králová B., Rauch P., Fukal L., Bioanalytická chemie, Vydavatelství VŠCHT Praha, 2007, ISBN 80-7080-449-1

R: Káš J., Kodíček M., Valentová O., Laboratorní techniky biochemie, Praha 2005, ISBN 80-7080-586-2

R: Ruml T., Rumlová M., Pačes V., Genové inženýrství, Praha 2002, ISBN 80-7080-499-8

R: Daussant J., Desvaux F.-X., Introduction to immunochemical techniques for medical diagnosis, food quality control and environmental testing, 2007, ICT Press, 9788070806418

Learning resources -
Last update: Kubová Petra Ing. (19.08.2019)

Additional list of suggested bibliography and electronic references will be provided within individual lectures.

Syllabus -
Last update: Kubová Petra Ing. (19.08.2019)

1. Bioanalytical methods definition, principle and practical applications.

2. Enzyme usage in analysis. Systematic classification of enzymes, enzyme kinetics.

3. Experimental techniques of enzyme methods. Determination of substrates and metabolites of enzyme reactions. Immobilized enzymes.

4. Application of enzyme methods in clinic analyses and food industry.

5. Imunochemistry -principle of immunochemical methods, antibodies, antigens.

6. Immunoprecipitation methods, application in practice

7. Sensitive immunochemical techniques (ELISA, immunoblot).

8. Methods with radionuclide application.

9. Methods based on molecular recognition. Bioafinity chromatography (incl. HAPLC), Imunosensors, FACS.

10. Electrophoretical methods:PAA,SDS, affinity electrophoresis, immunoelectrophoresis, Immunoblot. Capillary electrophoresis

11. Mass spectrometry in analysis of biomolecules.

12. Biological and microbiological methods

13. Genetical methods. Polymerase chain reaction, DNA and RNA array, fluorescence in situ, restriction analysis, DNA sequencing, application of genetical methods.

14. Detection limits and application of selected methods, Usage of modern analytical methods for biopolymers.

Registration requirements -
Last update: Svoboda Petr Ing. Ph.D. (18.11.2021)

Biochemistry I

Course completion requirements -
Last update: Kubová Petra Ing. (19.08.2019)

Oral examination