SubjectsSubjects(version: 901)
Course, academic year 2021/2022
Analysis of Bioactive Compounds - AM323016
Title: Analysis of Bioactive Compounds
Guaranteed by: Department of Food Analysis and Nutrition (323)
Actual: from 2021 to 2021
Semester: summer
Points: summer s.:4
E-Credits: summer s.:4
Examination process: summer s.:
Hours per week, examination: summer s.:2/1 C+Ex [hours/week]
Capacity: unlimited / unlimited (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
For type: Master's (post-Bachelor)
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Stránská Milena doc. Ing. Ph.D.
Dvořáková Darina Ing. Ph.D.
Incompatibility : M323016
Is incompatible with: M323016
This subject contains the following additional online materials
Last update: Dvořáková Darina Ing. Ph.D. (22.10.2021)
Within this course, principles and key applications of modern isolation, separation and detection techniques commonly used in the field of studies / characterization of biologically active compounds. Special attention is paid to (i) application of basic and advanced extraction techniques; (ii) effective separation strategy realized by gas / liquid chromatography; (iii) presentation of various instrumental techniques with a special focus on mass spectrometry and its presentation in case studies. In the final phase of course, practical aspects together with problems to be solved in a qualified manner in practice are summarized.
Aim of the course
Last update: Dvořáková Darina Ing. Ph.D. (22.10.2021)

Students will be able to understand:

  • How to make a qualified choice of suitable sample preparation strategy (i.e. extraction and clean-up technique) for determination of target analytes.

  • Principle and description of chromatographic techniques (gas or liquid chromatography) for the analysis of complex mixtures of biologically active compounds.

  • Characterization of mass spectrometry (ionization techniques, mass analyzers) and its use in conjunction with chromatography for identification and quantification of analytes.

  • Application of non-target screening (metabolomic fingerprinting / profiling) followed by statistical processing of experimental data.
Last update: Dvořáková Darina Ing. Ph.D. (09.11.2021)

Stauffer M. (ed.): Calibration and Validation of Analytical Methods, A sampling of Current Approaches (2018), e-source VŠCHT, ISBN: 978-1-78923-085-7; DOI: 10.5772/intechopen.69918

Naushad M. & Khan M.R. (ed): Ultra Performance Liquid Chromatography Mass Spectrometry, Evaluation and Applications in Food Analysis (2014), e-source VŠCHT, ISBN: 9780429073953;

Picó Y. (ed): Advanced Mass Spectrometry for Food Safety and Quality, Comprehensive Analytical Chemistry, Handboook, Volume 68, Pages 3-714 (2015), e-source VŠCHT, ISBN: 978-0-444-63340-8

Kusch P. (ed): Gas Chromatography, Derivatization, Sample Preparation, Application (2019), e-source VŠCHT, ISBN: 978-1-83881-866-1; DOI: 10.5772/intechopen.73339

Mutlu M. (ed): Biosensors in Food Processing, Safety, and Quality Control (2010); e-book: ISBN: 9780429104121; DOI: 10.1201/b10466

Beier R.C. & Stanker L.H. (ed): Immunoassays for Residue Analysis (1996); ISBN13: ‍9780841233799; DOI: 10.1021/bk-1996-0621

Learning resources
Last update: Dvořáková Darina Ing. Ph.D. (22.10.2021)

Group at MS teams, where students will complete short tests online to verify their knowledge at the end of each teaching block. After the evaluation of the tests, the results will be discussed with the students and the most common mistakes will be explained.

Technique overview:

Requirements to the exam
Last update: Kubová Petra Ing. (16.04.2018)

Within the extent of the syllabus.

Last update: Dvořáková Darina Ing. Ph.D. (22.10.2021)

1 Introduction to the issue, classification of biologically active substances

2 General characteristics of the analytical procedure, performance characteristics and validation, basic steps in the analysis of biologically active substances of both natural and anthropogenic origin

3 Basic and advanced extraction techniques with a focus on their use in practice

4 Gas chromatography (theory, injection techniques, classification of capillary columns, influence of various parameters on the separation process, optimization of separation, two-dimensional GC, conventional detectors)

5 Liquid chromatography (theory, selection of chromatographic system, columns and classification of stationary phases, conventional detectors, optimization and influence of various parameters on the separation process)

6 Mass spectrometry (introduction and characterization of application potential, ionization techniques, mass analyzers, ion mobility, detection)

7 Analysis of volatile compounds, GC-MS (case studies)

8 Analysis of environmental contaminants by GC-MS (case studies)

9 Analysis of environmental contaminants by LC-MS (case studies)

10 Bioanalytical methods I (biosenzors and portable systems for food analysis)

11 Bioanalytical methods II (principles of biomolecular recognition)

12 Analysis of pesticides and mycotoxins

13 Analysis of vitamins and other important biologically active compounds

14 Presentation of individual students works, discussion, preparation for final test

Registration requirements
Last update: Dvořáková Darina Ing. Ph.D. (22.10.2021)

Analytical chemistry, Food chemistry, Analysis of food and natural products

Course completion requirements
Last update: Dvořáková Darina Ing. Ph.D. (22.10.2021)

To obtain the credit, it is necessary to prepare a group project (3-4 students) in the form of a presentation, which summarizes several case studies in the analysis of a selected group of biologically active substances. The award of credit is a necessary condition for passing the exam, which consists of a written part and possibly an oral exam.

Teaching methods
Activity Credits Hours
Obhajoba individuálního projektu 0,5 14
Účast na přednáškách 1 28
Práce na individuálním projektu 1 28
Příprava na zkoušku a její absolvování 1 28
Účast na seminářích 0,5 14
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