SubjectsSubjects(version: 912)
Course, academic year 2022/2023
Analytical chemistry in biochemistry - M320002
Title: Analytická chemie v biochemii
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2020
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:0/2, MC [HT]
Capacity: unknown / unlimited (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type: Master's (post-Bachelor)
Guarantor: Kučková Štěpánka doc. Ing. Mgr. Ph.D.
Interchangeability : N320022
This subject contains the following additional online materials
Annotation -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (11.01.2018)
The course is focused on solving practical problems in the biochemical laboratory. The course summarizes the findings of analytical chemistry, biochemistry and biophysical chemistry.
Aim of the course -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (11.01.2018)

Students will be able:

To solve practical problems that commonly occurring in a biochemical laboratory.

Literature -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (11.01.2018)

R: Kodíček M., Karpenko V., Biofyzikální chemie, Academia, 2000, 80-200-0791-1

R: Káš J., Kodíček M., Valentová O., Laboratorní techniky biochemie, Vysoká škola chemicko-technologická v Praze, 2006, 80-7080-586-2

A: Churáček J. a kol., Analytická separace látek, SNTL, Praha 1990, 80-03-00569-8

Learning resources -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (11.01.2018)


Syllabus -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (11.01.2018)

1. Basic terms (measurements, quantities, characteristics of analytical methods), repetition of analytical calculations

2. Electroanalytical methods, preparation of distilled water, optical methods in biochemical laboratory

3. Biosensors, potentiometric sensors, the principles of pH measurements and maintenance of electrodes

4. Optical, thermal and piezoelectric transducers, sensors based on sensitive polymer layer, requirements for ideal biosensor

5. Procedures used for sample preparation for analysis, filtration, ultrafiltration, dialysis, sonication, microwave devices

6. Robotics, automation, system miniaturization

7. Separation methods, flow cytometry, centrifuges and centrifugal methods, rotors, field flow fractionation

8. Electrophoretic methods I: basic terms, separation modes

9. Electrophoretic methods II: electrophoretic titration curve, detection, blotting, processing and storage of electropherograms

10. Separation methods I: basic terms, methods, Van Deemter equation, TLC methods

11. Separation methods II: liquid chromatography, diagnostic of problems with chromatographic separation, gel chromatography

12. Detectors used in chromatography, derivatization of analytes, maintenance rules, selection of appropriate equipment

13. Vibrational spectroscopic methods - FTIR, Raman spectroscopy, immuno-SERS

14. Multidimensional separation techniques in proteomics, combining of different separation techniques and detectors

Registration requirements -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (11.01.2018)

Analytical Chemistry, Biochemistry, Biophysical Chemistry

Course completion requirements -
Last update: Kučková Štěpánka doc. Ing. Mgr. Ph.D. (11.01.2018)

Successful completion of the written examination.

Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0,5 14
Příprava na zkoušku a její absolvování 1,5 42
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