SubjectsSubjects(version: 863)
Course, academic year 2019/2020
  
Advanced Biochemistry: Laboratory - M320012
Title: Laboratoř speciální biochemie
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2019
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:0/5 MC [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type: Master's (post-Bachelor)
Guarantor: Lipov Jan Ing. Ph.D.
Novotná Zuzana doc. Dr. Ing.
Interchangeability : N320036
This subject contains the following additional online materials
Annotation -
Last update: Hladíková Jana (13.12.2017)
Tha aim of the subject is to expand the basic knowledge of the biochemical course and to adopt new techniques of modern biochemistry and to amend the spectrum of techniques of biochemistry and molecular biology used in research laboratories.
Aim of the course -
Last update: Hladíková Jana (13.12.2017)

Students will be able to:

isolate the proteins from various sources

characterize the enzyme activity

determine the degree of cooperativity of the enzyme during its denaturation

identify the protein by mass spectrometry

isolate plasmid DNA and characterize it by restriction cleavage

analyze and process the results to protocols

Literature -
Last update: Hladíková Jana (13.12.2017)

R: Káš J., Kodíček M., Valentová O.: Laboratorní techniky biochemie. 1. vyd. Vysoká škola chemicko-technologická v Praze, Praha 2006. ISBN 80-7080-586-2

Learning resources -
Last update: Hladíková Jana (13.12.2017)

Kodíček Milan: Biochemické pojmy : výkladový slovník (http://vydavatelstvi.vscht.cz/knihy/uid_es-002/)

Requirements to the exam -
Last update: Lipov Jan Ing. Ph.D. (25.01.2018)

Elaboration of protocols from all laboratory tasks.

Classification at least E from every protocol.

At least 50% from the final written test.

Syllabus -
Last update: Hladíková Jana (13.12.2017)

1. Good laboratory practice, safety

2. Theoretical introduction to the laboratory tasks

3. Chromatography of biomacromolecules, evaluation of purification steps

4. Electrophoretic analysis of erythrocyte membrane proteins

5. Basic characterization of purified preparates of proteins

6. Enzyme kinetics (allosteric enzymes)

7. Modern methods of protein identification

8. Physical & chemical properties of proteins

9. Immunotechniques

10.Basic techniques of molecular biology

11.Isolation of subcellular particles

12.Study of basic metabolic events

13.Methods of determination of catalytical activity of enzymes

14.Evaluation, test and free discussion

Registration requirements -
Last update: Lipov Jan Ing. Ph.D. (27.02.2018)

Biochemistry

Laboratory of biochemistry

Course completion requirements -
Last update: Novotná Zuzana doc. Dr. Ing. (25.01.2018)

Credit will be obtained based on a written solution of an individual task in given deadline. The exam comprises a written part.

Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1 28
Příprava na zkoušku a její absolvování 1 28
3 / 3 84 / 84
Coursework assessment
Form Significance
Report from individual projects 40
Continuous assessment of study performance and course -credit tests 60

 
VŠCHT Praha