SubjectsSubjects(version: 853)
Course, academic year 2019/2020
  
Genetic Engineering - M320017
Title: Genové inženýrství
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2019
Semester: both
Points: 3
E-Credits: 3
Examination process:
Hours per week, examination: 2/0 Ex [hours/week]
Capacity: winter:unlimited / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type: Master's (post-Bachelor)
Note: course can be enrolled in outside the study plan
enabled for web enrollment
you can enroll for the course in winter and in summer semester
Guarantor: Ruml Tomáš prof. Ing. CSc.
Interchangeability : AM320017, N320051
Is interchangeable with: AM320017
This subject contains the following additional online materials
Annotation -
Last update: Hladíková Jana (13.12.2017)
The subject is focused on understanding of principles of manipulation with nucleic acids and their analysis. It covers information about various methods of introducing genes into diverse cell types, determination of gene expression and analysis. The goal is to deliver information about genetic engineering techniques in order to facilitate the choce of the optimal method for a particular application. The lectures cover basic methods of isolation, analysis and modification of nucleic acids and special applications as gene modification for detection or affinity purification of gene product, study of interaction of proteins and nucleic acids, or gene therapies.
Aim of the course -
Last update: Hladíková Jana (13.12.2017)

Students will be able to:

Isolate DNA and perform basic manipulations with recombinant DNA. Prepare vectors for genetic modification of various cell types.

Modify and label DNA, apply DNA labeled probes for identification of specific sequences.

Modify genes by directed mutagenesis by directed mutagenesis, extension by sequences for detection and purification by affinity chromatography.

Analyze production of heterologous proteins and isolate them from various cell types.

Analyze protein interactions. Construct gene libraries.

Literature -
Last update: Hladíková Jana (13.12.2017)

R: Ruml T., Rumlová M., Pačes V.: Genové inženýrství; VŠCHT Praha 2002

R: Green M. R., Sambrook J., Molecular Cloning: A Laboratory Manual (Fourth Edition); Cold Spring Harbor Laboratory Press 2012

Learning resources -
Last update: Hladíková Jana (13.12.2017)

http://biomikro.vscht.cz/vyuka/?Predmet=gieng

Syllabus -
Last update: Ruml Tomáš prof. Ing. CSc. (13.09.2019)

1. Recapitulation of genetic principles.

2. Plasmid and viral vectors, artificial chromosomes.

3. DNA production and isolation, its analysis.

4. Restriction endonucleases, genome mapping,

5. Modification of nucleic acids for detection of specific sequences.

6. High-capacity methods of DNA sequencing.

7. Principles of work with RNA, subtraction libraries.

8. Application of polymerase chain reaction: product modification, controlled mutagenesis.

9. Protein production in microbial cells, fusion proteins, reporter genes.

10. Tissue cultures and their use for gene expression.

11. Principle of transgenic organisms preparation.

12. Detection of gene expression products - metabolic labeling, electrophoretic and immunochemical methods.

13. Gene therapy.

14. Methods for studying protein-nucleic acid interactions.

Registration requirements -
Last update: Kubová Petra Ing. (03.03.2018)

Biochemistry

Molecular genetics or Molecular genetics and DNA analysis

Course completion requirements -
Last update: Ruml Tomáš prof. Ing. CSc. (26.01.2018)

Passing written test with a score exceeding 50% and passing an oral exam

Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0,4 12
Příprava na zkoušku a její absolvování 1,3 35
3 / 3 75 / 84
Coursework assessment
Form Significance
Examination test 40
Oral examination 60

 
VŠCHT Praha