SubjectsSubjects(version: 922)
Course, academic year 2022/2023
Clinical Laboratory Diagnostics - M320029
Title: Klinická laboratorní diagnostika
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: 40 / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type: Master's (post-Bachelor)
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Fusek Martin prof. Ing. CSc.
Krausová Martina doc. Ing. Ph.D.
Interchangeability : N320011, N320011A
This subject contains the following additional online materials
Annotation -
Last update: Fusek Martin prof. Ing. CSc. (08.02.2018)
Students will learn about several areas in the course of Clinical Laboratory Diagnostics. The main topic is understanding of physiological and patho-physiological features of basic parameters with a focus on the relationship between biochemistry, physiology and a given disease.In the next part the methodological approaches used in human diagnostics will be explained. A detailed description of analyzed molecules, which are used in the current medicine according to the function of these molecules - such as enzymes, fats, hormones, etc will be given in the next part. For each category the function and the use for diagnostics will be explained. The last part of the study focuses on the diagnostics of liver, kidney, and the diagnosis of the internal environment of the organism, including acid-base balance.
Aim of the course -
Last update: Krausová Martina doc. Ing. Ph.D. (11.01.2018)

Students will be able to:

gain knowledge of the overall work flow of laboratories, quality control of analytical methods as well as other diagnostic laboratory methods used in medical practice.

Literature -
Last update: Kubová Petra Ing. (19.12.2017)

R:Schneiderka P. a kol., Stanovení analytů v klinické biochemii,1.část, Praha, Karolinum,1999, 80-7184-761-5

R:Schneiderka P. a kol., Kapitoly z klinické biochemie,Karolinum, 2004, 80-246-0678-X

A:Zima T. a kol.: Laboratorní diagnostika, třetí doplněné a přepracované vydání, Praha, Galén, 2013, 978-80-7262-372-3(Galén), 978-80-246-1423-6 (Karolinum)

A:Racek, J. a kol.: Klinická biochemie. Druhé, přepracované vydání Galén + Karolinum, Praha, 2006, 80-7262-324-9

A:Štern, P.a kol.: Obecná a klinická biochemie. Praha, Karolinum, 2011, 978-80-246-1979-8

A: Mačák J., Mačáková J. a Dvořáčková J.: Patologie. 2., doplněné vydání.

Grada Publishing 2012, 978-80-247-3530-6

Learning resources -
Last update: Kubová Petra Ing. (19.12.2017)


Syllabus -
Last update: Krausová Martina doc. Ing. Ph.D. (11.01.2018)

1. Introduction

Clinical biochemistry / definition, history, basic tasks / Overview of lectured themes / Basic materials analysed in a clinical laboratory / Quality biochemical analysis / Safety

2. Methods in Clinical Biochemistry I

Separation Methods / Analytical methods / Molecular genetic analysis / Molecular diagnostics

3. Methods in Clinical Biochemistry II

Immunochemical methods / Summarization of the testing - Basic investigated parameters / Specific tests

4. Metabolism of amino acids

Overview of amino acid / Amino acid metabolism / Metabolic disorders and their detection

5. Proteins and clinical biochemistry

Main types and functions of protein / Protein metabolism - synthesis and degradation / Examination protein / Main clinically significant plasma proteins / Proteins recovered in the urine

6. Enzymes - function and disorders

The role of enzymes in the body / Determination of enzymatic activity / Main enzymes in clinical biochemistry and their determination

7. Carbohydrate metabolism and its disorders

Carbohydrates and their metabolism / Physiological functions of carbohydrates / Diabetes and its examination / Other disorders of carbohydrate metabolism

8. Lipids and their metabolism

Structure and physiological functions / Digestion and transport / Apolipoproteins / Enzymes of lipid transport / Atherosclerosis / Biochemical screening

9. Liver functions and related biochemical screening

Liver - description and metabolism / Liver diseases / Analytical procedures for the diagnosis of liver diseases

10. Renal functions and related biochemical screening

Kidney - description and function / Main biomarkers for the diagnosis of renal functions / Analytical Methods

11. Hormones and their analysis

Classification and functions of basic hormone / Clinical applications

12. Tumor markers

Cancer diseases / Classification of tumor markers / Use of tumor markers

13. Electrolytes

Basic terms / Main monitored ions / Sodium and Potassium

14. Acid-base balance

Processes affecting pH / Role of carbon dioxide / pH control / ABB parameters

Entry requirements - Czech
Last update: Krausová Martina doc. Ing. Ph.D. (11.01.2018)

Znalost základů biochemie.

Registration requirements -
Last update: Krausová Martina doc. Ing. Ph.D. (11.01.2018)

Biochemistry I

Course completion requirements -
Last update: Fusek Martin prof. Ing. CSc. (25.01.2018)

Succesfully pass two written tests and the oral examination.

Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na zkoušku a její absolvování 2 56
3 / 3 84 / 84